| First Author | Becker-Follmann J | Year | 1992 |
| Journal | Cytogenet Cell Genet | Volume | 60 |
| Pages | 168 (Abstr.) | Mgi Jnum | J:2036 |
| Mgi Id | MGI:50560 | Citation | Becker-Follmann J, et al. (1992) Assignment of muscle cadherin (MCAD) to human Chromosome 16q24-qter and to mouse Chromosome 8, and of placental cadherin (PCAD) - like sequences PCADL1 to 16q22.1 and PCADL2 to 16q24-qter. Cytogenet Cell Genet 60:168 (Abstr.) |
| abstractText | Full text of abstract: Abstracts for the second international workshop on chromosome 16. Assignment of Muscle Cadherin (MCAD) to Human Chromosome 16q24-qter and to Mouse Chromosome 8, and of Placental Cadherin (PCAD) - Like Sequences PCADLl to 16q22.1 and PCADL2 to l6q24-qter. Becker-Follmann J1, Kaupmann K2, Vestweber D3, Jockusch H2, Starzinski-Powitz A4, Scherer G1. 1 Institute of Human Genetics, University of Freiburg, D-7800 Freiburg, FRG 2 Developmental Biology Unit, W7, University of Bielfeld, D-4800 Bielfeld, FRG 3 Max-Planck-Institute of Immunology, D-7800 Freiburg, FRG 4 Institute of Genetics, University of Koln, D-5000 Koln 1, FRG Cadherins are a group of at least four glycoproteins responsible for tissue-specific cell-cell adhesion. The loci for E-cadherin (uvomorulin, UVO) and N-cadherin (NCAD) have previously been assigned to human chromosomes 16q22.1 and 18, respectively (1, 2). We used cDNA clones for murine M-cadherin (Mcad) (3) and human P-cadherin (PCAD) for our mapping studies. The PCAD clone, isolated in a search for new members of the cadherin family, is a 2.4 kb cDNA clone, starting with bp 759 (according Shimoyama et al. (4)) and containing a poly A stretch at the 3' end. By Southern blot analysis using DNA from seven mouse x human somatic cell hybrids, the locus of the human homologue, MCAD, of mouse M-cadherin could be assigned to 16q24-qter, an interval to which APRT maps. Hybridization with the PCAD probe identifies a PCAD-like sequence, PCADLI, at 16q22.1, close to UVO, and an additional locus, PCADL2, at 16q24-qter. Very likely, PCADLl is the PCAD locus proper because the Pcad locus is closely linked to E-cadherin (uvomorulin, Um) and haptoglobin (Hp) on mouse chromosome 8(5). Using an interspecific backcross (6), we have mapped the mouse Mcad locus close to Aprt (no recombinants in 80 meioses), 7.5+/-2.9 cM distal to the E-cadherin (Um) locus. These results add two new loci for cadherins, in addition to the previously mapped E-cadherin (uvomorulin, UVO/Um) locus, to the conserved linkage group on HSAl6q and MMU8. 1 Natt E et al.: Cytogenet Cell Genet 50: 145-148 (1989). 2 Walsh FS et al.: J Neurochem 55: 805-812 (1990). 3 Donalies M et al.: Proc. Natl. Acad. Sci., USA 88: 8024-8029 (1991). 4 Shimoyama Y et al.: J Cell Bid 109: 1787-1794 (1989). 5 Hatta M. et al.: Nucl Acids Res 19: 4437-4441 (1991). 6 Kaupmann K et al,: Eur 1 Neurosci 3: 1182-1186 (1991). |
