| First Author | Mattei MG | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 657-658 (Abstr.) | Mgi Jnum | J:12348 |
| Mgi Id | MGI:60596 | Citation | Mattei MG, et al. (1987) In situ mapping of four cytotoxic T-lymphocyte associated proteins (CTLA) to the mouse genome. Cytogenet Cell Genet 46:657-658 (Abstr.) |
| abstractText | Full text of Abstract. Abstracts of workshop presentations. In situ mapping of four cytotoxic T-lymphocyte associated proteins (CTLA) to the mouse genome. Mattei MG,1 Harper K,2 Brunet JF,2 Denizot P,2 Mattei JF,1 Goldstein P,2 Giraud F.1 1INSERM U242 & Centre de Genetique Medicale, Hopital dÃEnfants de la Timone, Marseille. 2 Centre dÃImmunologie INSERM-CNRS de Marseille-Luminy, France. Study of the mechanism of T-cell-mediated cytotoxicity have now given way to a search for molecules involved in this function. These molecules may be identified by detecting the corresponding messenger RNA transcripts. Such an approach may include, as a first step, the search for transcripts as specific as possible to cytotoxic T-cells; only secondarily can their actual relevance to cytotoxicity be investigated. Four distinct messenger RNA transcripts (CTLA-1, CTLA-2, CTLA-3, CTLA-4) were detected by a systematic screening of a substracted complementary DNA bank. The corresponding genes were then mapped, bu in situ hybridization, on metaphase spreads from a WMP male mouse whose entire chromosome complement, other than the 19 and X chromosomes, is associated in the form of multiple robertsonian translocations (kindly give by JL Guenet, Pasteur Institute, Paris). The CTLA-1 gene was thus localized in the D band of mouse chromosome 14, in a region to which the T-cell receptor alpha-chain gene (TCRA) has been mapped. The CTLA-2 and CTLA-3 genes were assigned respectively to the B band and the D band of mouse chromosome 13. The CTLA-4 gene was assigned to the C band of mouse chromosome 1. |
