| First Author | Qin S | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 678 (Abstr.) | Mgi Jnum | J:12379 |
| Mgi Id | MGI:60627 | Citation | Qin S, et al. (1987) Mapping FSHB, CAT, and CTSB to specific sites on 11p. Cytogenet Cell Genet 46:678 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. Mapping FSHB, CAT, and CTSB to specific sites on 11p. Qin S,1 Nakai H,1 Byers MG,1 Eddy RL,1 Haley LL,1 Henry WM,1 Wang X,1 Watkins PC,2 Chirgwin JM,3 Shows TB.1 1Roswell Park Memorial Institute, Buffalo, NY. 2Integrated Genetics, Framingham, MA. 3University of Texas Health Science Center, San Antonio. The genes coding for the beta polypeptide of follicle-stimulating hormones (FSHB), catalase (CAT) and cathepsin D (CTSD) were regionally mapped using somatic cell hybrid deletion mapping and in situ hybridization techniques. Human-rodent somatic cell hybrids independently retaining three different WAGR (Wilms' tumor, aniridia, genitourinary, mental retardation syndrome) chromosome 11 deletions, with a combined smallest deleted region of 11pl3, were used to regionally map FSHB and CAT. Both FSHB and CAT were mapped to 11p in control cell hybrids but were not detected in the smallest deleted 11pl3 region using WAGR deletion hybrids. In the same way, CTSD was mapped to 11p13-qter using a chromosome 11 rearrangement and to 11pl5 using the WAGR deletion cell hybrids. Site-specific in situ hybridization mapping located FSHB within 11p13-p12, CAT to 11p13, and CTSD to 11p15. For FSHB, the majority of silver grains were located in pl3, and these tended to be located on the proximal side of 11p13. Together these somatic cell and in situhybridization data map FSHb to 11p13, CAT to 11p13, and CTSD to 11p15. HGM symbols : FSHB, CAT, CTSB |
