| First Author | de Gouyon B | Year | 1994 |
| Journal | Mouse Genome | Volume | 92 |
| Issue | 3 | Pages | 516-19 |
| Mgi Jnum | J:20790 | Mgi Id | MGI:68860 |
| Citation | de Gouyon B, et al. (1994) Fine genetic and physical mapping of thirteen mouse X Chromosome microsatellites. Mouse Genome 92(3):516-19 |
| abstractText | Full text of Mouse Genome contribution: FINE GENETIC AND PHYSICAL MAPPING OF THIRTEEN MOUSE X CHROMOSOME MICROSATELLITES. Beatrice de Gouyon, Aurobindo Chatterjee, and Gail E. Herman. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Tx. 77030 The region of the mouse X chromosome between coagulation factors IX (Cf9) and VIII (Cf8) is syntenic with human Xq27-q28. Numerous disease genes have been mapped to this region, some of which remain to be cloned (summarized in 1). Our laboratory has previously developed detailed genetic and physical maps of this region in the mouse, including a 2.3 Mb YAC contig spanning from Gabra3 to G6pd (2). Our interest in constructing these maps was primarily focused on the isolation of genes for two X-linked dominant, male lethal mouse mutations, bare patches (Bpa) and striated (Str) which map to this region (3) and may be homologous to human X-linked dominant chondrodysplasia punctata (CDPX2) and incontinentia pigmenti (IP), respectively. As part of efforts to isolate these mutations and to extend the physical maps of this region of the mouse X chromosome, we have attempted to further localize 15 microsatellite markers regionally mapped between Cf9 and Dmd (4). Genetic mapping utilized backcrosses involving Mus spretus (5, 6) or Mus castaneus (3) which were developed in our laboratory and have been typed for markers between Cf9 and Dmd. Two of these microsatellites, DXMit42 and DXMit112, were not polymorphic in either cross when tested against parental DNAs and were not studied further. Eleven markers demonstrated polymorphisms with M. spretus and two with M. castaneus (see Table 1). To map these thirteen markers, PCR primers were purchased from Research Genetics (Huntsville, Ala.), and DNA from recombinant mice from the appropriate cross was amplified under conditions described by Dietrich et al. (4). Three of the markers were further localized by physical mapping onto YACs in our growing contig between Ids and Dmd (2, and Chatterjee et al., manuscript in preparation). PCR amplifications were performed as above using YACs from the region. DXMit119 was positioned on YACs just proximal to Gabra3; DXMit59 on two overlapping YACs containing Llcam; and DXMit61 on a YAC obtained from Dr. A. Monaco, Oxford which contains the Dmd brain promoter. The microsatellite marker DXMit94, which cosegregated with DXMit119 and Gabra3, was not present on any of the YACs suggesting it maps proximal to these two loci. Similarly, DXMit44, 45, 60, and 77 lie within gaps between YACs at G6pd, Cf 8, and Dmd, and cannot be ordered with respect to each other. DXMit43 could only be typed in male recombinants since the M. spretus allele did not amplify well in heterozygous females. Although it cosegregated with Rsvp and Cf8 in the backcross, it was not found on any of our YACs, suggesting it lies within one of the gaps between G6pd and DXMit61. The positions of the 13 loci following our genetic and physical mapping are shown in Fig. 1. In summary, we have refined the positions for 13 microsatellite markers in the A7/B region of the mouse X chromosome. Our backcrosses which had been typed for many markers between Cf9 and Dmd enabled genetic localization, while YAC contigs in our possession for much of the region were used to further refine the map positions where possible. The microsatellites which do not identify existing YACs can now be used to extend our contigs. Those lying on one of the YACs provide additional highly polymorphic loci for a variety of mapping projects. References 1. Schlessinger, D. et al., (1993). Cytogenet. Cell Genet. 64:148-170. 2. Chatterjee, A. et al., (1994). Genomics 21:49-57. 3. Angel, T. et al., (1993). Mamm. Genome 4:171-176. 4. Dietrich, W. et al., (1992). Genetics 131:423-447. 5. Herman, G.E. and Walton, S. (1990). Genomics 7:307-312. 6. Herman, G.E. et al., (1991). Genomics 9:670-677. 7. Brown, S.D.M. et al., (1993) Mamm. Genome 4:S269-S281. Figure 1 (Legend). Schematic representation of the mouse X chromosome. The order and positions for the anchor loci shown were taken from (7). DXMit 43, 44, 45, 46, 59, 60, 61, 77, 94, 119, and 120 were typed in recombinant animals from a backcross of (B6CBA-Aw-J/A-Bpa) x Mus spretus (5, 6). DXMit25 and 110 were typed in progeny from a backcross between (C3H x 101) x Mus castaneus (3) (see Table 1). The dotted vertical lines in front of the loci DXMit110 and DXMit43 show the limits within which these markers must lie. The solid vertical lines to the right of the genetic map show the approximate extent of the YAC contig for this region. Distances within the expanded portion of the genetic map are drawn approximately to scale except where there are double slash marks. (Table 1 - Note.) B=C57BL/6Jaw-J allele, S=spretus allele, H=C3H/HeH allele, and C=castaneus allele inherited from the F1 heterozygous female. Ò?Ó indicates that typing results were ambiguous and not scored in that animal; "x" indicates a recombination event; and blank spaces indicate that the locus was not tested in that animal. |
