| First Author | Chakraborti A | Year | 1992 |
| Journal | Mouse Genome | Volume | 90 |
| Issue | 4 | Pages | 679-81 |
| Mgi Jnum | J:3459 | Mgi Id | MGI:51972 |
| Citation | Chakraborti A, et al. (1992) Microtubule-associated protein genes (Mtap-1, Mtap-2, Mtap-5) map to mouse chromosomes 1, 2 and 13. Mouse Genome 90(4):679-81 |
| abstractText | Full text of Mouse Genome contribution: MICROTUBULE-ASSOCIATED PROTEIN GENES (Mtap-1, Mtap-2, Mtap-5) MAP TO MOUSE CHROMOSOMES 1, 2 AND 13. A. Chakraborti and C.A. Kozak; National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892. Introduction: A number of proteins are found in association with tubulin in the mammalian brain. These microtubule associated proteins (MAPs) have apparent morphological functions in microtubule assembly and stability in axons and dendrites (reviewed in 1, 2). Characterization of these proteins reveals structural differences, although some MAPs share regions of homology in their microtubulin-binding domains (2, 3). Genes encoding MAP1, MAP2, and MAP5 have been cloned, and their use as hybridization probes has been responsible for the assignment of Mtap-1 to Chromosome (Chr) 2 and Mtap-5 to Chr 13 using somatic cell hybrids (4), and Mtap-2 to Chr 1 by the analysis of somatic cell hybrids and RI strains (5) . Here we report the chromosomal localization of all three of these genes using two multilocus crosses. Materials and Methods: Two multilocus genetic crosses were used for mapping: Cross 1 = (NFS/N or C58/J X M. m. muscu1us)Fl X M. m. musculus (6) and Cross 2 = (NFS/N X M. spretus)Fl X M. spretus or C58/J (7). DNAs extracted from the progeny of these crosses were typed for restriction enzyme polymorphisms using the protocol described by Hoggan (8) to permit separation of large fragments. Mice were typed using hybridization probes described previously for Mtap-1 and Mtap-5 (4) and for Mtap-2 (9) and kindly provided by Dr. C. Garner (Univ. Hamburg, Hamburg, Germany). Probes and RFLPs used to type progeny of the M. m. musculus cross were previously described as follows: Sfpi-1 and Hdc (10), Sag (11), and Il-9 and Dhfr (12). Progeny of the M. spretus cross were typed for RFLPs of these same markers with the following enzymes: Sfpi-1 and Hdc (EcoRI), Il-9 (HindIII) and Dhfr (PstI). Mice were also typed for Pcna following BglII digestion in both crosses using as probe a 1 kb fragment prepared by Dr. J. Hanley-Hyde (NCI, NIH, Bethesda, MD) from the genomic Pcna clone pGen-1 provided by Dr. P. Shipman -Appasamy (Pittsburgh Cancer Institute, Pittsburgh, PA). Idh-1 was typed by histochemical staining of kidney extracts on starch gels (13). Results and Discussion: The restriction enzyme fragment polymorphisms used to follow inheritance of Mtap-1, Mtap-2 and Mtap-5 in the two multilocus crosses are listed in Table 1. Inheritance of these polymorphisms was scored in the progeny of both crosses for Mtap-1 and Mtap-5, and in the M. m. musculus cross (Cross 1) for Mtap-2. Linkage was established for the three genes on Chr 1, 2, and 13 as indicated in Tables 2, 3, and 4. TABLE 1. Restriction enzyme fragment polymorphisms. Marker: Mtap-1; Enzyme: ScaI; M. m. musculus: 2.0; Fragment Sizes (kb) - NFS/N, C58/J: 9.4. Marker: Mtap-1; Enzyme: BglII; Fragment Sizes (kb) - NFS/N, C58/J: 10.5; M. spretus: 7.8. Marker: Mtap-2; Enzyme: ApaI; M. m. musculus: 12.2 or 18.0(a); Fragment Sizes (kb) - NFS/N, C58/J: 22.0. Marker: Mtap-5; Enzyme: ApaI; M. m. musculus: 12.4; Fragment Sizes (kb) Ð NFS/N, C58/J: 20.0. Marker: Mtap-5; Enzyme: BamHI; Fragment Sizes (kb) - NFS/N, C58/J: 10.5; M. spretus: 28.0. (a)Mtap-2 was polymorphic in the M. m. musculus colony used for breeding. These data confirm and extend previous studies which had mapped these genes using RI strains (Mtap-2) and somatic cell hybrids (Mtap-1, -2, -5) (4, 5). The human homolog of Mtap-2 (MAP2) has been mapped to chromosome 2q34-35 (14) consistent with the observation that proximal mouse Chr 1 contains a large region with linkage homology to human 2q31-qter (15). Similarly, assignment of Mtap-5 to distal Chr 13 suggests that the human homolog may reside on 5q11-13. The region of Chr 2 to which Mtap-1 has been assigned contains regions of linkage homology to human chromosomes 11, 6, 15, 2 and 20 (15). Genetic mapping of the human Mtap-1 homolog should help further define the karyotypic rearrangements among genes in this region which have occurred during mammalian evolution. References: 1. Matus, A. 1988. Ann. Rev. Neurosci. 11:29-44. 2. Goedert, M., et al. 1991. Trends Neurosci. 14:193-199. 3. Schoenfeld, T.A., et al. 1989. J. Neurosci. 9:1712-1730. 4. Garner, C.C., et al. 1990. J. Neurochem. 55:146-154. 5. Lafuse, W., et al. 1992. Mam. Genome 3:48-51. 6. Kozak, C.A., et al. 1990. Genomics 8:519-524. 7. Adamson, M.C., et al. 1991. Virology 183:778-781. 8. Hoggan, M.D., et al. 1983. J, Virol. 45:473-477. 9. Garner, C.C. and Matus, A. 1988. J. Cell Biol. 106:779-783. 10. Paul, R., et al. 1991. J. Virol. 65:464-467. 11. Danciger, M., et al. 1989. Genomics 5:378-381. 12. Mock, B.A., et al. 1990. Immunogenet. 31:265-270. 13. Harris, H. and Hopkinson, D.A. 1976. "Handbook of Enzyme Electrophoresis in Human Genetics", North Holland, Amsterdam. 14. Nave, R.L., et al. 1986. Mol Brain Res. 1:271-280. 15. Hillyard, A.L., et al. 1992. Mouse Genome 90:3-21. TABLE 2. Linkage of Mtap-1 to proximal Chr 2 markers. Inheritance of the allele of the F1 parent(a): Sfpi-1: +; Mtap-1: +; Hdc: +; Pcna: +; Number of Mice: Cross 1: 33; Cross 2: 31. Inheritance of the allele of the F1 parent(a): Sfpi-1: -; Mtap-1: -; Hdc: -; Pcna: -; Number of Mice: Cross 1: 27; Cross 2: 19. Inheritance of the allele of the F1 parent(a): Sfpi-1: +; Mtap-1: +; Hdc: +; Pcna: -; Number of Mice: Cross 1: 2; Cross 2: 1. Inheritance of the allele of the F1 parent(a): Sfpi-1: -; Mtap-1: -; Hdc: -; Pcna: +; Number of Mice: Cross 1: 0; Cross 2: 1. Inheritance of the allele of the F1 parent(a): Sfpi-1: +; Mtap-1: +; Hdc: -; Pcna: -; Number of Mice: Cross 1: 1; Cross 2: 0. Inheritance of the allele of the F1 parent(a): Sfpi-1: -; Mtap-1: -; Hdc: +; Pcna: +; Number of Mice: Cross 1: 1; Cross 2: 0. Inheritance of the allele of the F1 parent(a): Sfpi-1: +; Mtap-1: -; Hdc: -; Pcna: -; Number of Mice: Cross 1: 4; Cross 2: 3. Inheritance of the allele of the F1 parent(a): Sfpi-1: -; Mtap-1: +; Hdc: +; Pcna: +; Number of Mice: Cross 1: 5; Cross 2: 1. Gene order and recombination: Sfpi-1 - 10.1 +/- 2.7 - Mtap-1 - 1.6 +/- 1.1 - Hdc - 3.1 +/- 1.5 - Pcna. TABLE 3. Linkage of Mtap-2 to Chr 1 markers. Inheritance of the allele of the F1 parent(a): Idh-1: +; Mtap-2: +; Saq: +; Number of Mice - Cross 1: 26. Inheritance of the allele of the F1 parent(a): Idh-1: -; Mtap-2: -; Saq: -; Number of Mice - Cross 1: 22. Inheritance of the allele of the F1 parent(a): Idh-1: +; Mtap-2: +; Saq: -; Number of Mice - Cross 1: 3. Inheritance of the allele of the F1 parent(a): Idh-1: -; Mtap-2: -; Saq: +; Number of Mice - Cross 1: 1. Inheritance of the allele of the F1 parent(a): Idh-1: +; Mtap-2: -; Saq: -; Number of Mice - Cross 1: 0. Inheritance of the allele of the F1 parent(a): Idh-1: -; Mtap-2: +; Saq: +; Number of Mice - Cross 1: 1. Gene order and recombination: Idh-1 - 1.9 +/- 1.9 - Mtap-2 - 7.5 +/- 3.6 - Saq. TABLE 4. Linkage of Mtap-5 to distal Chr 13 markers. Inheritance of the allele of the F1 parent(a): Il-9: +; Dhfr: +; Mtap-5: +; Number of Mice: Cross 1: 42; Cross 2: 24. Inheritance of the allele of the F1 parent(a): Il-9: -; Dhfr: -; Mtap-5: -; Number of Mice: Cross 1: 54; Cross 2: 30. Inheritance of the allele of the F1 parent(a): Il-9: +; Dhfr: +; Mtap-5: -; Number of Mice: Cross 1: 2; Cross 2: 4. Inheritance of the allele of the F1 parent(a): Il-9: -; Dhfr: -; Mtap-5: +; Number of Mice: Cross 1: 5; Cross 2: 0. Inheritance of the allele of the F1 parent(a): Il-9: +; Dhfr: -; Mtap-5: -; Number of Mice: Cross 1: 7; Cross 2: 8. Inheritance of the allele of the F1 parent(a): Il-9: -; Dhfr: +; Mtap-5: +; Number of Mice: Cross 1: 15; Cross 2: 2. Gene order and recombination: Il-9 - 16.6 +/- 2.7 - Dhfr - 5.7 +/- 1.7 - Mtap-5. (a) Scored as the presence (+) or absence (- ) of the NFS/N or C58/J allele in Cross 1 (M. m. musculus cross) and the M. spretus allele in Cross 2 (M. spretus cross). |
