| First Author | Killary AM | Year | 1986 |
| Journal | Am J Hum Genet | Volume | 39 |
| Issue | Suppl | Pages | A159 (Abstr.) |
| Mgi Jnum | J:23181 | Mgi Id | MGI:71357 |
| Citation | Killary AM, et al. (1986) Assignment of the genes encoding dihydrofolate reductase and hexosaminidase B to mouse Chromosome 13. Am J Hum Genet 39(Suppl):A159 (Abstr.) |
| abstractText | Full text of Abstract: GENE MAPPING AND LINKAGE A159. (471) 20.67. ASSIGNMENT OF THE GENES ENCODING DIHYDROFOLATE REDUCTASE AND HEXOSAMINIDASE B TO MOUSE CHROMOSOME 13. A.M. Killary1, R.J. Leach2, R.G. Moran2, and R.E.K. Fournier2, 1Univ. of Texas Health Science Center at San Antonio, 2Univ. of Southern California School of Medicine. (Intro. by B.H. Bowman). The murine gene encoding dihydrofolate reductase [DHFR) has been localized to a particular mouse chromosome by complementation mapping. Microcells prepared from diploid mouse fibroblasts were fused with mutant hamster cells lacking the dihydrofolate reductase gene (Dhfr), and DHFR+ microcell hybrids were selected in medium lacking purines and pyrimidines. The complemented hybrids expressed wild-type levels of DHFR enzyme activity, and selectively retained a single mouse chromosome - chromosome 13. Genomic Southern blots of DNAs prepared from these hybrids and from an independently derived collection of clones isolated without selection for the DHFR+ phenotype confirmed the assignment of murine Dhfr gene sequences to chromosome 13. In man, the Dhfr gene has been assigned to the long arm of chromosome 5, syntenic with the loci encoding arylsulfatase B and hexosaminidase B. Information concerning the conservation and localization of this linkage group in the murine genome has been obtained only recently. The gene encoding arylsulfatase B has been assigned the mouse chromosome 13. Assignment of Dhfr to chromosome 13 provides further evidence for the existence of genetic homology between regions of mouse chromosome 13 and human chromosome 5. In support of this view, we have also mapped the gene encoding hexosaminidase B to mouse chromosome 13. |
