| First Author | Jones PP | Year | 1990 |
| Journal | Mouse Genome | Volume | 87 |
| Pages | 103-5 | Mgi Jnum | J:14292 |
| Mgi Id | MGI:62463 | Citation | Jones PP (1990) Recombinations in the H-2 - Upg-1 region of (H-2b x H-2d) F1 mice. Mouse Genome 87:103-5 |
| abstractText | Full text of Mouse Genome contribution: Research News: E.G. Spack; B. Kusler; P.P. Jones. Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020. Recombinations in the H-2- Upg-1 Region of (H-2b x H-2d)F1 Mice. In the course of studies on the genetic effects of in utero exposure to the folic acid antagonist methotrexate, we have detected 9 intra- H-2 recombinations and 47 recombinations between the TL region of the H-2 complex and the urinary pepsingen locus (Upg-1) located approximately 4cM telomeric to the TL region on chromosome 17. C57BL/10 SN (H-2b) and BALB/c J (H-2d) mice were mated, and the F1 progeny were exposed in utero on gestation day 11, 12, or 13 to 20 mg methotrexate /kg maternal body weight. F1 females were outcrossed to B10.A(4R) or BALB.K males to segregate the F1 chromosomes and permit screening of crossovers which occurred in the F1 (H-2b x H-2d) oocytes. The haplotypes of the parental and outcross strains are: Strain: C57BL/10 SN; Allele: K: b; D: b; Qa: b; TL: b; Upg-1: s. Strain: BALB/c J; Allele: K: d; D: d; Qa: d; TL: d; Upg-1: f. Strain: B10.A(4R); Allele: K: k; D: b; Qa: b; TL: b; Upg-1: s. Strain: BALB.K; Allele: K: k; D: k; Qa: k; TL: k; Upg-1: s. The H-2K and H-2D alleles were typed on peripheral blood leukocytes with radiolabelled monoclonal antibodies which did not cross react with the alleles of the outcross strain. This antibody screening identified recombinations within the MHC between the K and D loci (Table l). These recombinations may be useful for the analysis of haplotype specific recombinational hot spots in the I region. Recombination in the telomeric portion of the H-2 complex was identified by typing the electrophoretic variants of Upg-1 obtained from the urine of outcross mice (Szymurra, J.M., and Klein, J. Immunogenetics 13:267, 1981). Recombinants between the H-2D and Upg-1 loci were classified within the MHC (D-TL) or outside the MHC (TL-Upg-1) based on RFLP analysis of tail DNA from the outcross progeny with several intragenic probes derived from the Qa and TL regions. Five recombinants were mapped to the gap between the Qa and TL regions. Four of these recombinants are available as DNA samples or outcross progeny (Table 2). These recombinants might aid in the linkage of Qa and TL genes. Twenty-two samples are available with crossovers between the TL region and the Upg-1 locus (Table 3). These samples or mice may aid in the characterization of this segement of chromosome 17, including analysis of gene order in the Hmt region. We do not plan to maintain these recombinants in our mouse colony, but we would be happy to send mice or DNA samples to interested laboratories. Please be advised that quantities of DNA are limited, and attempts to discover RFLPs which distinguish the Hmt region of H-2b and H-2d with probes Mb-1 (Singer, D.S., et.al. Immunogenetics 28:13, 1988) and P7.500 (Richards, S., et.al. EMBO J. 8:3749, 1989) have been unsuccessful. Requests for material should be addressed to Dr. Patricia Jones at the address listed above. TABLE 1. K/D Recombinants. I.D. NO(2): 799M; ALLELE1: K(3): b; D: d; AVAILABILITY(4): D Ht Hm. I.D. NO(2): 2021C; ALLELE1: K(3): b; D: d; AVAILABILITY(4): D Ht Hm. I.D. NO(2): 2989M; ALLELE1: K(3): b; D: d; AVAILABILITY(4): D Ht. I.D. NO(2): 3038M; ALLELE1: K(3): b; D: d; AVAILABILITY(4): D Ht. I.D. NO(2): 2700M; ALLELE1: K(3): b; D: d; AVAILABILITY(4): D Ht. TABLE 2. Qa/TL Recombinants. I.D. NO.: 030M; ALLELE: D: b; tD/L(5): -; cQ8/9: b; cT17: d; cT11: -; tT10: d; 3ÃT18: d; Upg-1(6): d; AVAILABILITY: D. I.D. NO.: 156M; ALLELE: D: b; tD/L(5): b; cQ8/9: b; cT17: d; cT11: d; tT10: d; 3ÃT18: d; Upg-1(6): d; AVAILABILITY: D Ht Hm. I.D. NO.: 371M; ALLELE: D: b; tD/L(5): b; cQ8/9: b; cT17: d; cT11: d; tT10: d; 3ÃT18: d; Upg-1(6): d; AVAILABILITY: D. I.D. NO.: 587M; ALLELE: D: d; tD/L(5): d; cQ8/9: d; cT17: b; cT11: b; tT10: b; 3ÃT18: b; Upg-1(6): b; AVAILABILITY: D Hm. TABLE 3. TL/Upg-1 Recombinants. I.D. NO.: 011C; ALLELE: D: b; tD/L: -; cQ8/9: b; cT17: -; cT11: -; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 034M; ALLELE: D: b; tD/L: -; cQ8/9: b; cT17: b; cT11: -; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 055M; ALLELE: D: b; tD/L: -; cQ8/9: b; cT17: b; cT11: -; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 120M; ALLELE: D: b; tD/L: -; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 128M; ALLELE: D: b; tD/L: -; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 153M; ALLELE: D: b; tD/L: -; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 163M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: b; cT11: -; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D Ht Hm. I.D. NO.: 186M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: -; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 345C; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 352C; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D Ht Hm. I.D. NO.: 363M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D Ht Hm. I.D. NO.: 411M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 512M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 517M; ALLELE: D: b; tD/L: b; cQ8/9: -; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D. I.D. NO.: 544M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D Ht Hm. I.D. NO.: 558M; ALLELE: D: b; tD/L: b; cQ8/9: b; cT17: -; cT11: b; tT10: b; 3ÃT18: b; Upg-1: d; AVAILABILITY: D Ht Hm. I.D. NO.: 255M; ALLELE: D: d; tD/L: -; cQ8/9: d; cT17: d; cT11: -; tT10: d; 3ÃT18: d; Upg-1: b; AVAILABILITY: D. I.D. NO.: 421M; ALLELE: D: d; tD/L: d; cQ8/9: -; cT17: -; cT11: d; tT10: d; 3ÃT18: d; Upg-1: b; AVAILABILITY: D Ht Hm. I.D. NO.: 519M; ALLELE: D: d; tD/L: d; cQ8/9: d; cT17: -; cT11: d; tT10: d; 3ÃT18: d; Upg-1: b; AVAILABILITY: D. I.D. NO.: 526M; ALLELE: D: d; tD/L: d; cQ8/9: d; cT17: -; cT11: d; tT10: d; 3ÃT18: d; Upg-1: b; AVAILABILITY: D. I.D. NO.: 572M; ALLELE: D: d; tD/L: d; cQ8/9: d; cT17: -; cT11: d; tT10: d; 3ÃT18: d; Upg-1: b; AVAILABILITY: D. I.D. NO.: 601M; ALLELE: D: d; tD/L: d; cQ8/9: d; cT17: -; cT11: d; tT10: d; 3ÃT18: d; Upg-1: b; AVAILABILITY: D. 1 Only the alleles of the recombinant chromosome are listed. Mice 2021C, 2989M, 3038M and 2700M are outcross progeny of F1(C57BL/10 x BALB/c) females with BALB.K males. All others listed in Tables 1-3 are the progeny of F1(C57BL/10 x BALB/c) females outcrossed to B10.A(4R) males. 2 M indicates exposure of the F1 female parent to methotrexate during its fetal development; C indicates that the F1 female was part of the control group and was not exposed to methotrexate during its fetal development. 3 K and D alleles were typed with monoclonal antibodies B8-24.3 (anti H-2Kb), SF1-1.1 (anti H-2Kd), MA66 (anti H-2Db) and 34-5.8S (anti H-2Dd). 4 D indicates DNA samples are available; Ht indicates mice that are available heterozygous for the chromosome bearing the recombination; Hm indicates that mice are available homozygous for the recombination (not on a congenic background). 5 Qa and Tl region haplotypes were typed by RFLP analysis with a series of intragenic probes hybridizing to fragments centromeric (c) or telomeric (t) to the indicated genes. Cosmid clones were from the BALB/c library described by Winoto, A., Steinmetz, M., and Hood, L. Proc. Natn. Acad. Sci. U.S.A. 80:3425 (1983). LOCATION: tD/L; PROBE: 0.6 Kb Bgl II fragment 4 Kb 5Ã of H-2Lk (p14913-1); b/d HAPLOTYPE RFLP: Hind III. LOCATION: cQ8/9; PROBE: 2.9 Kb Cla I/Sma I fragment of cosmid 46.1; b/d HAPLOTYPE RFLP: Hind III. LOCATION: cT17; PROBE: 2.6 Kb Xho I/Hpa I fragment of cosmid 47.1; b/d HAPLOTYPE RFLP: Kpn I. LOCATION: cT11; PROBE: 2.0 Kb Bam HI fragment of cosmid 22.1; b/d HAPLOTYPE RFLP: Hind III. LOCATION: tT10; PROBE: 2.2 Kb Eco RI fragment of cosmid 12.2; b/d HAPLOTYPE RFLP: Eco RI. LOCATION: 3ÃT18; PROBE: 4.5 Kb Eco RI/Hind III fragment of cosmid 15.3 (pMT21); b/d HAPLOTYPE RFLP: Kpn I. 6 Upg-1 alleles were typed electrophoretically: BALB/c (H-2d) = Upg-1f; C57BL/10 (H-2b) = Upg-1s; all outcross progeny had one chromosome bearing Upg-1s from the B10.A(4R) or BALB.K parent. |
