| First Author | Ashworth A | Year | 1987 |
| Journal | Mouse News Lett | Volume | 79 |
| Pages | 84 | Mgi Jnum | J:14134 |
| Mgi Id | MGI:62309 | Citation | Ashworth A, et al. (1987) Chromosomal localization of mouse cDNAs homologous to the Drosophila gene Kruppel. Mouse News Lett 79:84 |
| abstractText | Full text of MNL contribution: The gap gene Kruppel is one of the genes responsible for the development of the correct segmental pattern of the early Drosophila embryo (Nusslein-Volhard and Weischaus, Nature 287, 283, 1980). Kruppel has been shown to code for a protein of 466 amino acids which contains four putative DNA binding fingers homologous to those previously described in the Xenopus TFIIIA gene (Rosenberg et al., Nature 319, 336, 1986). In order to identify mammalian homologous of Kruppel, we have used a probe derived from the finger region (kindly supplied by H. Jackle via R. Cortese) to screen cDNA libraries in gamma gt10 made from 8.5 day post coitum mouse embryos (kindly supplied by B. Hogan) and mouse testis. Two hundred thousand recombinants were screened in duplicate from each library. Two independent cDNAs were isolated from the embryo library and one from the testis library. DNA sequence analysis demonstrated that each cDNA contained multiple putative DNA-binding fingers homologous to those present in the Drosophila Kruppel gene. All three sequences appear to be different to the two mouse genomic sequences described by Chowdhury et al (Cell 48, 771, 1987). Using the BXD series of recombinant inbred strains, we have determined the chromosomal localization of the two embryo-derived sequences, mouse Finger Protein (Fnp-1) and mouse Finger Protein 2 (Fnp-2). The Fnp-1, Fnp-2 strain distribution pattern is shown below. Fnp-1 - 1:B; 2:D; 5:B; 6:D; 8:B; 9:B; 11:B; 12:B; 13:D; 14:B; 15:D; 16:D; 18:B; 19:D; 20:B; 21:D; 22:B; 23:B; 24:B; 25:B; 27:B; 28:D; 29:D; 30:B; 31:B; 32:D. Fnp-2 1:D; 2:B; 5:D; 6:D; 8:D; 9:D; 11:D; 12:B; 13:D; 14:B; 15:B; 16:D; 18:D; 19:B; 20:D; 21:D; 22:B; 23:B; 24:D; 25:D; 27:D; 28:B; 29:D; 30:D; 31:B; 32:D. Computer analysis of these distributions provisionally assigns these sequences to mouse chromosome 11 ( F M ) and 8 (Fnp-2). Further analysis of the Fnp-1 pattern by the method of Silver (J. Hered. 76,436, 1985) suggests the following possible gene order for chromosome 11 : Hba - (A8, Fnp-1) - Sparc. Studies are in progress to identify the function of these finger proteins. |
