| First Author | Davisson MT | Year | 1979 |
| Journal | Mouse News Lett | Volume | 60 |
| Pages | 50-1 | Mgi Jnum | J:13795 |
| Mgi Id | MGI:61974 | Citation | Davisson MT, et al. (1979) [Four-point backcross on Chr 7.]. Mouse News Lett 60:50-1 |
| abstractText | Full text of MNL contribution: Chr 7. 1. A four point back cross involving markers on Chr 7 gave the following recombination fractions and percentages. The data are separated according to the sex of the heterozygous parent. Loci: Gpi-1 to ru-2; Females: 49/404 = 12.1 +/- 1.6%; Males: 21/181 = 11.6 +/-2.38% Loci: ru-2 to Mod-2; Females: 82/404 = 20.3 +/- 2.0%; Males: 29/181 = 16.0 +/- 2.7% Loci: Mod-2 to Hbb; Females: 26/404 = 6.4 +/- 1.2%; Males: 4/181 = 2.2 +/- 1.1% Some of these animals actually contained another segregating locus, Tam-1 (Skow, Genetics, in press) which show Tam-1 to be between Gpi-1 and ru-2. These data are independent of Skow's published data and show the following recombinations: Loci: Tam-1 to ru-2; Females: 5/165 = 3.0 +/- 1.3%; Males: 0/71 = 0.0% We had hoped these crosses would yield double crossovers which would provide information on interference. Double crossovers did occur much as one would expect except for an excess in region ru-2 to Hbb where we might expect none at all. We suspect these particular recombinations can be attributed to some kind of error, possibly the mixing of labels on samples. These samples were therefore not included. The study emphasizes the importance in studies of interference characterizing the alleles at all loci on living animals which can be retested and bred. (Davisson, S.H. Langley, Roderick, and L.C. Skow) Dr. Skow is now at Oak Ridge National Laboratory. |
