| First Author | Prochazka M | Year | 1991 |
| Journal | Mouse Genome | Volume | 89 |
| Pages | 553 | Mgi Jnum | J:23849 |
| Mgi Id | MGI:71726 | Citation | Prochazka M, et al. (1991) Mapping of Iapp and Kap genes to Chr. 6. Mouse Genome 89:553 |
| abstractText | Full text of Mouse Genome contribution: 2. Mapping of Iapp and Kap genes to Chr 6. During our ongoing studies of the predisposition to spontaneous insulin-dependent diabetes in nonobese diabetic (NOD/Lt) mouse strain, we established a linkage of the islet amyloid polypeptide gene (Iapp) and the kidney androgen-regulated protein gene (Kap) with Ly-49 on the distal part of Chr 6. Using cDNA probes for Kap (kindly provided by Dr. G. Watson, The Jackson Laboratory), and Iapp (kindly provided by Dr. G. Bell, University of Chicago, Chicago) for southern blot analysis of DNA from NOD/Lt and nonobese normal (NON/Lt) mice, we identified allelic variants between both strains (Iapp/PstI fragment: NOD-20 kb, NON-7.5 kb; Kap/DraI fragment: NOD-8.3 kb, NON-7 kb). Segregation analysis of 22 [(NOD x N0N)Fl x NOD] BCl offspring revealed no recombination of Iapp and Kap with Ly-49, indicating a close linkage of these loci. Ly-49 maps to a region of Chr 6 marked by Prp, Tpi-1, and Gapd, that is homologous with human 12p13. Human IAPP has been also positioned on 12p, and our Iapp data expand the homologous region in the mouse (Prochazka and Leiter). Results: Kap: NOD; F1. Iapp; NOD; F1; Ly-49; NOD; F1. N; 10; 12. |
