| First Author | Shows TB | Year | 1989 |
| Journal | Cytogenet Cell Genet | Volume | 51 |
| Pages | 1079 (Abstr.) | Mgi Jnum | J:30720 |
| Mgi Id | MGI:78484 | Citation | Shows TB, et al. (1989) Localization of the human hexokinase I gene (HK1) to Chromosome 10q22. Cytogenet Cell Genet 51:1079 (Abstr.) |
| abstractText | Localization of the human hexokinase I gene (HK1) to chromosome 10q22. (A2233) TB Shows1, RL Eddy1, MG Byers1, LL Haley1, WM Henry1, S. Nishi2, GI Bell2. 1Roswell Park Memorial Institute, Human Genetics Department, 666 Elm St., Buffalo, NY 14263; 2Howard Hughes Medical Institute, The University of Chicago, 5841 S. Maryland Ave., Box 391, Chicago, IL 60637. Mammalian hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) comprise a family of glucose phosphorylating enzymes which includes hexokinase I, II and III. In 1974, we demonstrated synteny of the gene coding for hexokinase to the gene coding for glutamic oxaloacetic transaminase using biochemical assays and human-mouse somatic cell hybrids (Shows et al., 1974). These genes were subsequently assigned to human chromosome 10. Recently, we have isolated cDNA clones encoding human hexokinase I (Nishi et al., 1988). This cDNA hybridized to human DNA fragments and co-segregated with human chromosome 10 in a panel of somatic cell hybrids. A chromosome 10 deletion in this panel localized HK1 to the pter-q23 region of this chromosome. In situ hybridization to human prometaphase chromosomes confirmed this regional assignment and further localized HK1 to 10q22. |
