| First Author | Post LC | Year | 1995 |
| Journal | 9th International Mouse Genome Conference | Pages | 87 (Abstr. 75) |
| Mgi Jnum | J:32747 | Mgi Id | MGI:80235 |
| Citation | Post LC, et al. (1995) Genetics of vertebrate digital arch formation. 9th International Mouse Genome Conference :87 (Abstr. 75) |
| abstractText | Full text of Abstract: 75. GENETICS OF VERTEBRATE DIGITAL ARCH FORMATION. L.C. Post1, D.P. Mortlock1, S. Darling3, J.W. Innis1,2. Department of Human Genetics1, Department of Pediatrics2, University of Michigan, Ann Arbor, MI, USA; University College, London, UK3. Organization of prechondrogenic condensations in the developing limb occurs via three main mechanisms: de novo condensation, bifurcation of single elements, and segmentation of existing mesenchyme. Perturbation of any of these events during development will lead to limb abnormalities. Therefore, investigation of natural and induced mouse mutations affecting the limb will lead to the identification of some of the genetic determinants mediating these processes. Hypodactyly (Hd) is a semidominant mutation in mice that maps to mouse chromosome six near the Hoxa locus. Homozygous affected mice fail to form the digital arch. Our goal is to examine the mechanisms responsible for digital arch formation in vertebrates by identification and analysis of the normal and affected gene product(s) at the Hd locus. Towards this goal, we have built a high-resolution genetic map of the Hd locus using genetic crosses with CASA/Rk and Mus spretus involving over 1,500 mice. We have identified markers nonrecombinant with the locus, oriented the Hoxa complex on the chromosome, and positioned Hd distal to Hoxa2 . We have constructed a contig of genomic clones which spans the proximal breakpoint and extends distal to Hoxa and the Evxl gene. We are currently testing the possibility of allelism between Hd and the 5' Hoxa genes and Evx1. |
