| First Author | Goto T | Year | 1997 |
| Journal | Genet Res | Volume | 70 |
| Pages | 79 (Abstr.) | Mgi Jnum | J:44119 |
| Mgi Id | MGI:1099370 | Citation | Goto T, et al. (1997) Regulation of the Xist minimal promoter studied by microinjection in preimplantation embryos and in transgenic mice. Genet Res 70:79 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of papers presented at the seventh Mammalian Genetics and Development Workshop held at the Wellcome Trust Building, Euston Road, London on 20-22 November 1996. Regulation of the Xist minimal promoter studied by microinjection in preimplantation embryos and in transgenic mice. TETSUYA GOTO, ELISABETH CHRISTIANS and MARILYN MONK Molecular Embryology Unit, Institute of Child Health, 30 Guilford Street, London WC1N IEH, UK The mouse Xist gene is expressed only from an inactive X chromosome and plays a role in X-chromosome inactivation in female development and in male spermatogenesis. Differential expression of the paternal Xist in preimplantation development has also been implicated in imprinted paternal X-inactivation in extra-embryonic tissues. To identify important regulatory elements in the Xist promoter, we have made a construct ligating a 233 bp PCR-amplified Xist promoter fragment (-220 to + 13) to the firefly luciferase reporter gene and the SV40 enhancer. Following injection of the construct into 1-cell embryos, luciferase activity is detected at the 2-cell stage. Methylation of the construct by site-specific methylases before injection results in a decrease in luciferase activity. We have also created transgenic lines harbouring the 2-5 kb linearized Xist-luciferase insert. In all six lines established, luciferase is highly expressed in the testis. In contrast, only background luciferase activity is observed in the liver, kidney, muscle, brain and ovary. A DNA methylation study shows that the transgene is undermethylated in the testis compared with other tissues examined. The regulatory sequences involved in promoter activation, imprinting and tissue-specificity are being investigated. |
