| First Author | Baker WH | Year | 1992 |
| Journal | Exp Hematol | Volume | 20 |
| Pages | 771 (Abstr.) | Mgi Jnum | J:1291 |
| Mgi Id | MGI:49821 | Citation | Baker WH, et al. (1992) Comparison of interleukin-1 alpha (IL-1) gene expression in murine spleens after lethal and sublethal 60Co irradiation. Exp Hematol 20:771 (Abstr.) |
| abstractText | Full text of Abstract. 256. COMPARISON OF INTERLEUKIN-1 ALPHA (IL-1) GENE EXPRESSION IN MURINE SPLEENS AFTER LETHAL AND SUBLETHAL 60Co IRRADIATION. Baker WH, Limanni A, Chang CM, Williams JL, Patchen ML, Armed Forces Radiobiology Research Institute, Bethesda, MD, USA. IL-1 alpha and beta have been demonstrated to be radioprotective when administered before irradiation. In addition, irradiated mice treated with anti-interleukin-1 receptor antibody exhibit decreased survival further implicating IL-1 in radiation resistance. To further evaluate the role of IL-1 alpha in radioprotection, we have investigated IL-1 messenger ribonucleic acid (mRNA) expression in irradiated mice. B6D2F1 female mice were given either a sublethal 7.75 Gy or lethal 9.75 Gy bilateral 60Co exposure (0.4 Gy/min), and spleens were harvested at 5 minutes, and 2, 4, 6, 8, 10, 12, and 24 hours later. Total cellular RNA was processed and 0.5 ug samples per time point were reverse-transcribed. The resultant complementary deoxyribonucleic acid (cDNA was amplified for 20 cycles via the polymerase chain reaction (PCR), and PCR products were Southern blotted and hybridized with 32P-labeled probe. Radiogrphic images were scanned and results expressed as densitometric volumes. In both sublethally and lethally irradiated mice IL-1 alpha mRNA expression at 5 minutes postirradiation was undetectable and only minimally detectable at 2 hours postirradiation. At later time points, IL-1 alpha mRNA expression in spleens from lethally irradiated mice displayed a 7- to 12-fold increase above normal expression, while expression in sublethally irradiated mice increased 2- to 3-fold over these same time points. Our results suggest that postirradiation IL-I alpha mRNA expression increases with increasing radiation exposure. In order to establish if increased expression of mRNA ultimately translates into increased protein production at these time points, immunohistochemical and serological studies will be done. |
