| First Author | Ashley PL | Year | 1987 |
| Journal | Am J Hum Genet | Volume | 46 |
| Pages | A155 (Abstr.) | Mgi Jnum | J:12140 |
| Mgi Id | MGI:60391 | Citation | Ashley PL, et al. (1987) Chromosomal assignment of the murine Gi and Gs genes. Am J Hum Genet 46:A155 (Abstr.) |
| abstractText | Full text of Abstract. Chromosomal assignment of the murine Gi and Gs genes. P.L. Ashley, J. Ellison, K.A. Sullivan, H.R. Bourne, and D.R. Cox. University of California, San Francisco. The G protein family of transmembrane signaling molecules includes Gs and Gi, the stimulatory and inhibitory regulators of adenylate cyclase. We used cloned cDNA probes representing the murine Gialpha and Gsalpha mRNAs in conjunction with a panel of Chinese hamster-mouse somatic cell hybrids segregating mouse chromosomes to map the Gialpha and Gsalpha genes in the mouse. Our results indicate that the Gialpha locus maps to mouse chromosome 9, while Gsalpha is localized to region 2E1-2H3 of mouse chromosome 2. These results represent the first mapping of G protein alpha chain genes in any species. Previous results suggested that the autosomal recessive mouse mutation obese (ob), which results in an abnormal response of adipose tissue to lipolytic hormones, is due to a defect in the gene coding for the alpha chain of Gi. Localization of the Gialpha gene to chromosome 9 excludes this gene as a site of the ob mutation, since the ob locus maps to chromosome 6. A human genetic disorder, pseudohypoparathyroidism, characterized by hormone resistance, short stature, brachydactyly, and decreased mental function, is caused by a generalized partial deficiency of Gs activity. Assignment of the murine Gsalpha gene to chromosome 2 facilitates the search for mutants in this locus, which may provide a model for further study of pseudohypoparathyroidism and contribute to our knowledge of this signal transduction apparatus. |
