| First Author | Miyajima A | Year | 1992 |
| Journal | Exp Hematol | Volume | 20 |
| Pages | 752 (Abstr.) | Mgi Jnum | J:1290 |
| Mgi Id | MGI:49820 | Citation | Miyajima A, et al. (1992) Expression of the beta subunits of the murine IL-3, GM-CSF and IL-5 receptors. Exp Hematol 20:752 (Abstr.) |
| abstractText | Full text of Abstract: 181. EXPRESSION OFTHE Beta SUBUNITS OF THE MURINE IL-3, GM-CSF AND IL-5 RECEPTORS. Atsushi Miyajima*, Dan Gorman*, and Takahiko Hara.* DNAX Research Institute, Palo Alto, CA, USA. High affinity receptors for IL-3, GM-CSF and IL-5 consist of alpha and Beta subunits. Whereas the alpha subunits are specific for each cytokine, the mouse has two homologous Beta subunits: one (BetaIL3, or AIC2A) is specific for the IL-3 receptor and the other (BetaC, or AIC2B) is common among the three receptors. We studied the expression of these Beta subunits. Two Beta subunits were coexpressed in various hemopoietic cells. Characterization of the chromosomal genes has revealed that the two Beta subunit genes were closely linked on the same chromosome and the exon-intron structure as well as the surrounding sequences was well conserved between the two genes. These results indicate that the two Beta subunit genes arose by a duplication. Conservation of the 5' upstream regions of the two genes that contained several potential binding sites for the hemopoietic transcription factors including GATA-1 and PU.1 suggest that a common mechanism regulates the transcription of the two genes. In addition, we have found a novel mechanism that dominantly suppresses the expression of both Beta subunits by destabilizing the Beta subunit mRNAs. This mechanism has a potential to shut off the response to IL-3, GM-CSF and IL-5 simultaneously. |
