| First Author | Naylor SL | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 669 (Abstr.) | Mgi Jnum | J:12368 |
| Mgi Id | MGI:60616 | Citation | Naylor SL, et al. (1987) Lactoferrin maps to human Chromosome 3(q21-q23) and mouse Chromosome 9. Cytogenet Cell Genet 46:669 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. Lactoferrin maps to human chromosome 3(q21-q23) and mouse chromosome 9. Naylor SL, Marshall A, Solomon A, McGill JR, McCombs J, Magnuson VL, Moore CM, Lalley PA, Pentecost PT, Teng C. University of Texas Health Science Center, San Antonio, TX. Institute for Medical Research, Bennington, VT. National Institute of Environmental Health Sciences, Research Triangle Park, NC. Lactotransferrin is an extracellular iron binding that is related to, but distinct from transferrin. A mouse cDNA for lactoferrin was used in the mapping of both mouse and human lactoferrin. A BstEII digest of DNA from human-mouse hybrids was used to identify a 20 kb fragment of human DNA which segregated in the hybrid clones. Southern filter analysis of 20 hybrid clones placed the LTF gene on chromosome 3. Hybrid segregating portions of chromosome 3 placed the LTF gene in the 3q21-qter region. Analysis of 57 metaphases by in situ hybridization of the lactoferrin probe localized the gene to 3q21q23 (76% of the grains that were located on chromosome 3). To compare the gene location of lactoferrin in mouse and human, we also mapped the lactoferrin gene using mouse-Chinese hamster hybrids. A HindIII digest of mouse and Chinese hamster DNA was used to detect 3 fragments of 10.6, 4.4, and 3.1 kb which segregated in the hybrids. In 12 mouse-Chinese hamster hybrids the mouse LTF sequences are located on chromosome 9. The transferrin gene is also on mouse chromosome 9, and in humans, both lactoferrin and transferrin are in the same region of chromosome 3. Thus it appears lactoferrin and transferrin are in a conserved synteny group. Because of the high degree of homology between transferring and lactoferrin (~80% at the amino acid level), it is possible that these two markers may be very close to each other. HGM symbol: LTF |
