| First Author | Barton DE | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 577 (Abstr.) | Mgi Jnum | J:4879 |
| Mgi Id | MGI:53360 | Citation | Barton DE, et al. (1987) The myelin-associated glycoprotein (GMA) gene maps to human Chromosome 19 and mouse Chromosome 7. Cytogenet Cell Genet 46:577 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. The myelin-associated glycoprotein (GMA) gene maps to human chromosome 19 and mouse chromosome 7. Barton DE,1 Arquint M,2 Roder J2,3 Dunn R,3 Francke U.1. 1Department of Human Genetics, Yale University School of Medicine, New Haven, CT. 2Division of Molecular Immunology & Neurobiology, Mt Sinai Hospital Research Institute, Toronto, Ontario, Canada. 3Department of Medical Genetics, University of Toronto, Ontario, Canada. Myelin-associated glycoprotein (GMA), a low-abundance membrane protein present in the myelin of both central and peripheral nervous systems, is thought to play a role in the formation of the myelin sheath. A MAG cDNA clone (pMAG1.2) has been isolated from a rat brain cDNA library, and expression studies revealed that MAG mRNA is most abundant at the time of active myelination in rat brain (Arquint et al. Proc Natl Acad Sci USA 84:600, 1987). The chromosomal location of the human GMA gene was determined by Southern blot analysis of DNA from 20 rodent x human hybrid cell lines using the pMAG 1.2 probe. A single hybridizing fragment of 17kb was detected by this probe on Southern blots of HindIII-digested human DNA and in all hybrids which had retained human chromosome 19. To determine the chromosomal location of this gene in the mouse, the same pMAG 1.2 probe was used on a panel of 11 Chinese hamster x mouse somatic cell hybrid DNAs digested with EcoRI. pMAG1.2 detected a major EcoRI fragment of 5.0kb in mouse DNA and in all hybrids which had retained mouse chromosome 7. All other mouse chromosomes were excluded as possible sites for MAG by at least three discordant hybrids. The region of mouse chromosome 7 that is homologous to human chromosome 19 also carries quivering (qv), the locus for a neurological mutation. Whereas MAG-specific DNA restriction fragments, mRNA and protein from qv/qv mice were apparently normal in size and abundance, we have not ruled out the possibility that qv could be caused by a point mutation in the MAG gene. HGM symbol: GMA |
