| First Author | Dudley K | Year | 1994 |
| Journal | Genet Res | Volume | 63 |
| Issue | 2 | Pages | 149 (Abstr.) |
| Mgi Jnum | J:18594 | Mgi Id | MGI:66857 |
| Citation | Dudley K, et al. (1994) TCP-11, a protein encoded within the mouse t-complex, is expressed in elongating spermatids. Genet Res 63(2):149 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of papers. TCP-11, a protein encoded within the mouse t-complex, is expressed in elongating spermatids KEITH DUDLEY, RAMINE HOSSEINI, SUZANNE RUDDY, SRINTHER BAINES AND PHILIP MARSH Developmental Biology Research Centre. Randall Institute, King's College, London The t-complex on mouse chromosome 17 is known to influence male fertility in two major ways. Mice which carry both copies of chromosonle 17 in the t-haplotype form are often partly or completely sterile. Mice which are heterozygous and carry one normal chromosome 17 and one in the t-haplotype form display transmission ratio distortion (TRD). We have isolated a gene, Tcp-11, which maps to the distal inversion of the t-complex and is a candidate for a gene involved in TRD. The gene is first expressed towards the end of meiosis in secondary spermatocytes and it codes for a 62 kD novel protein of unknown function. Analysis of the predicted amino acid sequence of the protein reveals an RGD sequence and a leucine heptad repeat. We have expressed Tcp-11 in E. coli, purified the protein and used it to raise polyclonal antisera in rabbits. On sections of testis the antisera recognise elongating spermatids with the staining being predominantly cytoplasmic. On Western blots of protein prepared from highly purified populations of germ cells a strong signal is detected in elongating spermatids with a weaker signal in round spermatids. |
