| First Author | Dudley K | Year | 1993 |
| Journal | J Reprod Fertil | Volume | 11 |
| Pages | 21 (Abstr.) | Mgi Jnum | J:13194 |
| Mgi Id | MGI:61400 | Citation | Dudley K, et al. (1993) TCP-11, a protein encoded within the mouse t-complex, is expressed in elongating spermatids. J Reprod Fertil 11:21 (Abstr.) |
| abstractText | Full text of Abstract: TCP-11, a protein encoded within the mouse t-complex,is expressed in elongating spermatids. Keith Dudley, Ramine Hosseini, Suzanne Ruddy, Srinther Baines and Philip Marsh. Developmental Biology Research Centre, Randall Institute, King's College, London WC2B 5RL The t-complex on mouse chromosome 17 is known to influence male fertility in two major ways. Mice which carry both copies of chromosome 17 in the t-haplotype form are often partly or completely sterile. Mice which are heterozygous and carry one normal chromosome 17 and one in the t-haplotype form display transmission ratio distortion (TRD). Depending on the haplotype these mice can transmit their sperm harbouring the t-haplotype chromosome with an efficiency of up to 95%, rather than the expected 50%. We have previously isolated a gene,Tcp-l1,which maps to the distal inversion of the t-complex and is a candidate for a gene involved in TRD. The gene is first expressed towards the end of meiosis in secondary spermatocytes and it codes for a 62kD novel protein of unknown function. Analysis of the predicted amino acid sequence of the protein reveals an ROD sequence and a leucine heptad repeat. We have expressed Tcp-11 in E. Coli, purified the protein and used it to raise a polyclonal antisera in rabbits. On thin sections of testis the antisera recognises elongating spermatids with the staining being predominantly cytoplasmic. On western blots of protein prepared from highly purified populations of germ cells ;i strong signal is detected in elongating spermatids with a weaker signal in round spermatids. No specific signal could be detected in spermatocytes or other tissues. Constructs are being made to test the function of this gene in transgenic animals. |
