| First Author | Barton DE | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 577 (Abstr.) | Mgi Jnum | J:4880 |
| Mgi Id | MGI:53361 | Citation | Barton DE, et al. (1987) The genes for ribophorins I and II are on human Chromosomes 3q and 20 and mouse Chromosomes 6 and 2, respectively. Cytogenet Cell Genet 46:577 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. The genes for ribophorins I and II are on human chromosomes 3q and 20 and mouse chromosomes 6 and 2, respectively. Barton DE,1 Crimaudo C,2 Hortsch M,2 Francke U.1. 1Department of Human Genetics, Yale University School of Medicine, New Haven, CT, 2European Molecular Biology Laboratory, Heidelberg, FRG. The ribophorins are two abundant glycoproteins characterized by their presence only in the membranes of the rough endoplasmic reticulum. It has been postulated that these two proteins may be involved in ribosome binding. Human cDNAs for both ribophorins I and II have recently been isolated from a liver expression library and sequenced. They are not related to each other at the primary sequence level (Crimaudo et al. EMBO J 6:75, 1987). They were used to map their cognate loci by Southern blot analysis of DNA from a panel of somatic cell hybrids. The ribophorin I cDNA hybridized with five fragments of 8.0, 4.7, 2.7, 1.6 and 1.2kb in PstI digests of human DNA and all hybrids retaining human chromosome 3. Furthermore, the ribophorin I sequences were present in hybrids containing the long arm of chromosome 3 (3q) but were absent in hybrids containing the short arm (3p), in the absence of the intact chromosome. The ribophorin II cDNA hybridized with seven EcoRI fragments of 13.5, 7.4, 3.5, 2.3, 1.5, and 1.3kb in human DNA and all hybrids which contained human chromosome 20. In both cases, all other chromosomes were ruled out as possible sites of either gene by at least five discordancies. In the mouse, Southern blot analysis of somatic cell hybrid DNA was used to assign the locus for ribophorin I gene to MMU6 and the ribophorin II gene to MMU2. Southern blotting studies to examine the conservation of the ribophorin genes through evolution demonstrated that both human ribophorin cDNAs hybridized with all mammalian DNAs tested and with Xenopus laevis DNA, while the ribophorin I cDNA also hybridized with yeast DNA sequences. We suggest locus names of RPN1 and RPN2 for ribophorins I and II, respectively. HGM symbol: RPN1, RPN2 |
