| First Author | Esworthy S | Year | 1981 |
| Journal | Mouse News Lett | Volume | 64 |
| Pages | 90 | Mgi Jnum | J:13885 |
| Mgi Id | MGI:62063 | Citation | Esworthy S, et al. (1981) Seminal vesicle protein variants. Mouse News Lett 64:90 |
| abstractText | Full text of MNL contribution: Seminal vesicle protein variants. The secretion of the C57BL/6J strain seminal vesicle contains 6 major protein families resolvable on 2d gels. These are SVSP-1 (2 proteins 14,000 daltons, pIs 5.3 and 5.6), SVSP-2 (2 or 3 proteins 35,000 daltons, pIs 6.6-7.0), SVSP-3 (2-3 proteins 16,000 daltons, pIs 7.0-7.2), SVSP-4 (2 proteins 14,500 daltons, pIs 7.8-8.0), SVSP-5 (2 proteins 35,000 daltons, pI 10.0, 38,500 daltons, pI 9.5), SVSP-7 (1 protein 85,000 daltons, pI 5.0). SVSP-5 is the major clottable protein of the secretion. The SVSP-1 group is variant among inbreds as described by Platz and Wolfe (J. Hered. 60, 187-192, 1969). The locus that controls the variation is called Svp-1 and maps near the Agouti locus on chromosome 2. Svp-la mice have a more basic doublet than B6 by about 0.3 pH units and the proteins are 200-500 daltons smaller. We surveyed wild mice for new variants. Variants for SVSP-3 were found in M. caroli, M. spretus, M.m. molossinus, M.m. danish. Variants for SVSP-5 were found in M. caroli, M. spretus, and M.m. california. Svp-1 alleles seen in wild mice conformed to the Svp-la or Svp-1b found in inbred strains. M. spretus in our colony is uniformly Svp-la and all members of the colony have a variant SVSP-3 and SVSP-5 proteins. The SVSP-3 protein in M. spretus is ~13,500 daltons with a pH of 7.6. The SVSP-5 protein of M. spretus has a molecular weight of 30,000. The Fl (B6 X M. spretus) shows codominant expression of all B6 and M. spretus SVSPs. Backcrosses to B6 and M. spretus show that the SVSP-3 and SVSP-5 phenotypic differences between B6 and M. spretus map as a single locus inseparable from Svp-1. Recombination of 4% with the Agouti locus was observed in these crosses. We are pursuing the possibility that the seminal vesicle secretory protein structural genes are clustered at Svp-1. The structural genes encoding the seminal vesicle mRNA that direct synthesis of the major in vitro translation products may also have a chromosome 2 assignment. This was deduced from studies with cloned cDNA made from seminal vesicle mRNA used to probe for mouse gene sequences in hamster-mouse cell lines segregating mouse chromosomes. We believe the major in vitro translation products are the secreted proteins. The major in vitro translation products show variation between strains of mice consistent with this hypothesis. Studies with in vitro translations of seminal vesicle mRNA from mice spanning 15 days t o 55 days post-partum suggests that while the mRNAs for some major products are abundant and fairly constant in cellular concentration over this period the mRNA for the in vitro product showing a strong resemblance to SVSP-1 is strongly induced beginning at 25-30 days post-partum. (S. Esworthy and K.W. Gross with P.A. Lalley, Oak Ridge). |
