| First Author | Lamb JG | Year | 1992 |
| Journal | FASEB J | Volume | 6 |
| Issue | 5 | Pages | A1671 (Abstr.) |
| Mgi Jnum | J:675 | Mgi Id | MGI:49210 |
| Citation | Lamb JG, et al. (1992) Cloning of mouse Ugtla-1 and rabbit UGT1A1 UDP glucuronosyltransferase cDNAs. FASEB J 6(5):A1671 (Abstr.) |
| abstractText | Full text of Abstract: 4252. CLONING OF MOUSE Egtla-1 and RABBIT UGT1A1 UDP GLUCURONOSYLTRANSFERASE cDNAs. J.G. Lamb, and R.H. Tukey. Departments of Pharmacology and Medicine, UCSD Cancer Center, La Jolla, CA, 92093. UDP glucuronosyltransferases (UDPGTs) are a family of enzymes involved in the elimination of endogenous (ex; biliruben and steroids) and exogenous compounds (ex; drugs, xenobiotics, and carcinogens). Analysis of UGT1A cDNAs reveals that the 3Õ regions are conserved while the 5Õ regions are divergent. Treatment of animals with 3-methylcholanthrene (3MC) and 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) induces UGT1A-1. We have isolated the PAH inducible Ugt1a-1 cDNA from mouse liver mRNA and UGT1A1 cDNA from rabbit liver mRNA. The mouse Ugt1a-1 cDNA was cloned using a 5' oligonucleotide and a 3' anti-sense oligonucleotide made to the previously isolated rat liver UGT1A-1 (JBC, 261:15607, 1986). The 3Õ anti-sense oligonucleotide was used in a reverse transcriptase reaction with mRNA from 3MC treated mouse liver, followed by a polymerase chain reaction (PCR) to amplify a 1.6 Kb cDNA fragment. The rabbit UGT1A1 cDNA was cloned from a rabbit liver lambda-ZAP library as a 2.1 Kb insert using a 350 bp fragment of DNA from the mouse Ugt1a-1 clone, specific for the 5' non-conserved region of the mRNA. DNA sequence analysis of the 5' region to date shows that the mouse Ugt1a-1 clone is 90% and 92% similar to the rat UGT1A1 cDNA and protein sequences respectively. However, the rabbit UGT1A1 clone is 80% and 73% similar to the rat UGT1A1 cDNA and protein sequences respectively. Northern blot analysis of mouse mRNA indicates that the Ugt1a-1 mRNA is present constitutively but is induced by treatment with 3MC or TCDD. (Supported by USPHS Grant CA37139). |
