| First Author | Konyukhov BV | Year | 1992 |
| Journal | Mouse Genome | Volume | 90 |
| Issue | 3 | Pages | 426 |
| Mgi Jnum | J:2514 | Mgi Id | MGI:51037 |
| Citation | Konyukhov BV, et al. (1992) Study of the mutant mi gene expression in mouse aggregation chimeras. Mouse Genome 90(3):426 |
| abstractText | Full text of Mouse Genome contribution: Research News: Study of the mutant mi gene expression in mouse aggregation chimeras. B.V. Konyukhov, N.A. Malinina, E.G. Marchuk. By aggregation of eight-cell embryos of two mouse strains we have produced 17 chimeras of 30-day old: 3 mi/mi <--> +/+, 9 mi/+ <--> +/+ and 5 +/+ <--> +/+ . Chimerism was identified by mosaicism in coat colour and retinal pigment epithelium (RPE). All mi/mi <--> +/+ chimeras had microphthalmia: eye disturbance was similar to that of the mi/mi eyes. The lack of the vitreous body led to the folded retina, which was in the contact with the lens and often had no rods. In these three chimeras RPE and choroid were mosaic: pigmented patches interspersed with unpigmented patches. Mosaicism of the RPE might be a result of an influence of paraorbital mesenchyme cells of different genotypes: +/+ or mi/mi. The pigmented patches RPE often overlying the pigmented patches of the choroid were cuboidal resembling normal RPE. The findings have confirmed earlier obtained data in our laboratory, that mutant alleles at the mi locus affect ectomesenchyme cells (neural crest cells) which play an important role in normal RPE development. The findings indicate that the mi gene acts in melanoblasts, but the mi/mi dermal and epidermal cells do not suppress proliferation or differentiation of +/+ melanoblasts. The mi/mi dermal cells also do not disturb the expression of the A gene. In spite of the high percent of mi/mi cells (58-80%) in mi/mi <--> +/+ chimeras the normal bone resorption and tooth eruptionhave been observed. |
