| First Author | Mullins LJ | Year | 1987 |
| Journal | Mouse News Lett | Volume | 77 |
| Pages | 150-151 | Mgi Jnum | J:14120 |
| Mgi Id | MGI:62296 | Citation | Mullins LJ, et al. (1987) Assignment and linkage of four new genes to the mouse X chromosome. Mouse News Lett 77:150-151 |
| abstractText | Full text of MNL contribution: Assignment and linkage of four new genes to the mouse X chromosome. A set of recombinant animals informative for murine mapping studies has been generated by mating males of the wild mouse stocks Mus musculus (Denmark) and Mus spretus with females of the inbred strain C57BL/6J and backcrossing F1 females with C57BL/6J males. We have identified restriction fragment length polymorphisms (RFLPs) for several X-linked genes in DNA of the wild and inbred parental stocks and have followed the segregation of RFLPs in the Fl generation to verify X chromosome linkage. Segregation of RFLPs in the B1 generation has been scored to estimate recombinational distances and determine gene order. To date, recombination frequencies have been established for ornithine transcarbamylase (Otc), tissue inhibitor of metalloproteinases (Timp), hypoxanthine phospshoribosyl transferase (Hprt), glucose-6-phosphate dehydrogenase (G6pd), coagulation factor IX (Cf-9), coagulation factor VIII (Cf -8), red sensitive visual pigment (Rsvp) and -galactosidase (Ags). The proposed gene order for these loci is: centromere, Otc, Timp, Hprt, Cf-9, (Gbpd, Cf-8, Rsvp), Ags. In the Denmark backcross study no recombinants have been found between Otc and Timp in 73 male offspring examined. Similarly no recombinants between G6pd, Cf-8 and Rsvp have been observed in 52 male offspring. By using different recombinant-X congenic strains, however, it has been possible to identify a recombinant between Otc and Timp thus enabling us to establish the gene order described above. As the wild-derived Mus species used in our work differs from laboratory strains for all genes so far investigated, it should be possible to use RFLPs to map other X-chromosome genes as and when the probes become available. (L.J. Mullins, S.G. Grant, J. Pazik, D.A. Stephenson and V.M. Chapman) |
