| First Author | Maruyama N | Year | 1991 |
| Journal | Mouse Genome | Volume | 89 |
| Pages | 278 | Mgi Jnum | J:14307 |
| Mgi Id | MGI:62478 | Citation | Maruyama N, et al. (1991) Restriction length fragment polymorphism in mouse peptidylarginine deiminase gene (Pdi). Mouse Genome 89:278 |
| abstractText | Full text of Mouse Genome contribution: RESTRICTION LENGTH FRAGMENT POLYMORPHISM IN MOUSE PEPTIDYLARGININE DEIMINASE GENE (Pdi). Naoki Maruyama, Setsuko Handa, and Kazutada Watanabe+; Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173 and +Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka 820 Japan. Peptidylarginine deiminase (PAD; EC3.5.3.15) is an enzyme which converts the guanido group of arginine residues in proteins to the ureido group of citrulline residues. We have reported previously that there are at least three types of PAD in mammalian tissues (1). Recently, we have cloned the cDNA (lambda PAD3) encoding the rat PAD to analyze its function (2). By using the cDNA of rat PDA, we examined the genetic constitution of mouse PAD gene. We have found the crosshybridization of rat cDNA (lambda PAD3) with mouse genomic DNA, and a restriction fragment length polymorphism (RFLP) in EcoRI digested genomic DNAs (Figure 1). We have designated the locus encoding mouse PAD as Pdi. This locus has two alleles among the strains tested (Table 1). 1. Watanabe, K., Akiyama, K., Hikichi, K., Ohtsuka, R., Okuyama, A. and Senshu, T. (1988) Biochim. Biophys. Acta, 966:375. 2. Watanabe, K. and Senshu, T. (1989) J. Biol. Chem. 264:15255. Figure 1. (Legend). RFLP using lambda PAD3 in C57BL/6 (B) and DBA/2 (D) mice. Table 1. Strain Distribution of Pdi Alleles in Inbred Strains* Allele: b; EcoRI fragment (kb): 25.5; Strains: A/J, BXSB, C57BL/6, C57BL/10, B10.D2; EcoRI fragment (kb): 20.0; Strains: CBA/J, C3H/HeN, NZB, NZW. Allele: d; EcoRI fragment (kb): 28.0; Strains: AKR/J, DBA/2, MRL/lpr, NC/Jic, ddY, ICR; EcoRI fragment (kb): 9.5. *Allele designations were made by Southern hybridization of endonuclease-digested DNA from the designated mouse kidney. DNA was digested with EcoRI and probed with AD3. |
