| First Author | Bobey NAM | Year | 1995 |
| Journal | Mouse Genome | Volume | 93 |
| Issue | 2 | Pages | 431-2 |
| Mgi Jnum | J:26239 | Mgi Id | MGI:73918 |
| Citation | Bobey NAM, et al. (1995) Strain distribution pattern for purine nucleoside phosphorylase (Np) in the AXB and BXA Recombinant Inbred strains. Mouse Genome 93(2):431-2 |
| abstractText | Full text of Mouse Genome contribution: STRAIN DISTRIBUTION PATTERN FOR PURINE NUCLEOSIDE PHOSPHORYLASE (Np) IN THE AXB AND BXA RECOMBINANT INBRED STRAINS. Nicola A.M. Bobey and Floyd F. Snyder; Departments of Medical Genetics and Medical Biochemistry, University of Calgary, Calgary, Alberta T2N 4N1 Canada. INTRODUCTION The sequencing of four alleles at the purine nucleoside phosphorylase locus in the mouse (1) resulted in the withdrawal of the two locus classification, Npl and Np2, and replacement with the single locus Np (1,2). Mice of the C57 and C58 background are Npb, whereas most other inbred strains including as we shall show, A/J, are Npa. Two other alleles represented by M.m. molossinus, Npc, and M. spretus, Npd, were also described (1). We report here the molecular typing of Np for the AXB and BXA recombinant inbred strains using genomic DNA. MATERIALS AND METHODS DNA. Mouse DNA was purchased from the Jackson Laboratory as prepared from: A/J, C57BL/6J, the 20 AXB and 18 BXA recombinant inbred strain sets. Molecular Analysis. PCR amplification of genomic DNA was performed essentially as previously described using primers 5'Np and 3'Np which give an 800 bp product (1,3). Taq I digestion of amplified DNA results in 195 and 605 bp fragments for products of the Npa allele, whereas the PCR product of Npb allele is refractory to digestion (1,3). RESULTS AND DISCUSSION An 800 bp PCR product was obtained upon amplification of A/J and C57BL/6J DNA with nucleoside phosphorylase specific primers 5'Np and 3'Np. Digestion of the 800bp product of A/J DNA with Taq I gave 195 and 605 bp products (Fig. 1) as previously observed for C3H/HeHa, C3H/He.J and DBA/2J DNA characteristic of the Npa allele (1,3). The C57BL/6J 800 bp PCR product was refractory to digestion characteristic of the Npb allele (Fig. 1). The strain distribution pattern for the AXB and BXA recombinant inbred sets are shown in Figure 1 and summarized in Table 1. Of the 20 AXB strains typed, 19 are identical to the pattern previously obtained by enzyme electrophoresis (4). Strain 21 previously designated C57BL/6J like was shown to have the A/J allele. Three new strains were typed in the BXA series, strains 11, 18 and 26. Of the remaining 15 BXA strains, only strain 4 differed from the previous classification. In the case of both AXB strain 21 and BXA strain 4, the present findings eliminate a previous crossover between Np and the adjacent loci Tcra (5,6) and Xmv19 (7). Table 1. Np locus strain distribution pattern for the AXB and BXA recombinant inbred strain sets. AXB STRAINS 1: B; 2: A; 3: B; 4: A; 5: B; 6: A; 7: A; 8: B; 9: A; 10: B; 11: B; 12: -; 13: Bl 14: Bl 15: Bl 16: -; 17: B; 18: B; 19: B; 20: B; 21: A; 22: ; 23: (Note: A Ð Figure 1 shows strain 23 as A). BXA STRAINS 1: B; 2: -; 3: -; 4: B; 5: -; 6: -; 7: A; 8: A; 9: B; 10: -; 11: B; 12: B; 13: A; 14: A; 15: -; 16: A; 17: A; 18: A; 19: -; 20: A; 21: -; 22: A; 23: A; 24: A; 25: A; 26: A. Figure 1. (Legend) PCR-RFLP analysis of the AXB and BXA recombinant inbred strain sets and the progenitor strains for purine nucleoside phosphorylase. An 800 bp fragment was amplified from genomic DNA and the Taq I site present in the Npa allele, A/J, is distinguished from its absence in the Npb allele, C57BL/6J. DNA molecular-weight marker VII (Boehringer Mannehim) was present in the outside lane. ACKNOWLEDGEMENTS This work was supported by grant MT-6376 from the Medical Research Council of Canada. 1. Jenuth, J.P., Mangat, R.K., Snyder, F.F. (1993) Mammalian Genome 4, 598-603. (15202) 2. Nadeau, J.H., McCarthy, L., Cox, R. (1994) Mammalian Genome 5, S207-S216. 3. Jenuth, J.P. and Snyder, F.F. (1993) Mouse Genome 91, 873-875. 4. Nesbitt, M., Pugh, S.L., Paigen, B. (1990) Mouse Genome 88, 113. 5. Traystman, M.D., Chow, L.H., McManus, B.M., Herskowitz, A., Nesbitt, M.N., Beisel, K.W. (1991) Am. J. Pathol. 138, 721-726. 6. Nadeau, J.H. and Cox, R.G. (1992) Mammalian Genome 3, S206-S219. 7. Frankel, W.N., Stoye, J.P., Taylor, B.A., Coffin, J.M. (1989) J. Virol. 63, 1763-1774. |
