| First Author | Timms GP | Year | 1982 |
| Journal | Mouse News Lett | Volume | 66 |
| Pages | 61 | Mgi Jnum | J:13906 |
| Mgi Id | MGI:62084 | Citation | Timms GP, et al. (1982) Microsomal aldehyde dehydrogenase (Ahd-3r - a new regulatory locus). Mouse News Lett 66:61 |
| abstractText | Full text of MNL contribution: Microsomal aldehyde dehydrogenase (Ahd-3r - a new regulatory locus). Variation in the inducibility of the 1iver microsomal isozyme of aldehyde dehydrogenase (designated AHD-Cy) by phenobarbital admini stration was observed among inbred strains and Harwell linkage testing stocks. Previous genetic analyses (Timms and Holmes, Genetics 97: 327-336) have shown that AHD-Cy is genetically distinct to the large granular (AHD-A2; Ahd-1 locus on chromosome 4) and the cytoplasmic (AHD-B4; Ahd-2 on chromosome 19) isozymes. The phenotypes were inherited in a normal Mendelian fashion with two alleles showing codominance at a proposed regulatory locus (designated Ahd-3r). A/J and BALB/c strains exhibited no microsomal AHD induction by phenobarbital treatment under the conditions used (0.5 mgm/ml for 3 days followed by 1 mgm/ml for 3 days in drinking water) whereas 101/H, C3H/He, C57BL/6J and linkage stocks LII and LVI exhibited 3 fold increases in activity. Backcross analyses of F1 (BALB/c x LII) and F1 (BALB/c x LVI) hybrids to both parents in each case supported the proposal for the regulatory locus. Electrophoretic analyses of microsomal AHD from induced and uninduced animals demonstrated that a single form of activity was present. (Timms and Holmes) |
