| First Author | Kozak LP | Year | 1988 |
| Journal | Mouse News Lett | Volume | 82 |
| Pages | 127 | Mgi Jnum | J:14228 |
| Mgi Id | MGI:62400 | Citation | Kozak LP, et al. (1988) Molecular characterization of a novel gene upstream from the glycerol-3-phosphate dehydrogenase gene in mouse. Mouse News Lett 82:127 |
| abstractText | MOLECULAR CHARACTERIZATION OF A NOVEL GENE UPSTREAM FROM THE Full text of MNL contribution: GLYCEROL-3-PHOSPHATE DEHYDROGENASE GENE IN MOUSE. Leslie P. Kozak and Leslie A. Johnston. The Jackson Laboratory, Bar Harbor, Maine. During the course of our studies on characterizing the glycerol-P dehydrogenase gene (Gdc-1, located on chromosome 15, we observed that the transcriptional unit for another gene was located a mere 3.4 kb upstream of the start site for Gdc-1 transcription. Since we do not know the function of this gene it has been named D15Kzl as recommended by the committee for nomenclature for such DNA regions. Unique sequence probes of D15Kzl hybridize to RNA species of 3.2 and 3.4 kb in length. Certain tissues such as brown fat express the 3.2 kb RNA, the cerebellum expresses the 3.4 kb form, while the cerebral cortex expresses both forms. Similar to the Gdc-1 gene D15Kzl is expressed in all adult tissues examined, however D15Kzl is also expressed in undifferentiated cell cultures unlike Gdc-1. Although both genes are ubiquitously expressed, there appears to be no co-ordinate expression. The cDNA sequence of a nearly complete D15Kzl cDNA clone has been shown to have an open reading frame of 461 amino acids. Using the dFASTp and dFASTn programs to search the protein and nucleic acid data bases we have not detected significant sequence similarity to any other gene or protein. Southern blot analysis of genomic DNA using the D15Kzl cDNA as a probe indicates that at least one additional copy of the gene is present in the mouse genome. Furthermore the D15Kzl probe hybridizes to low copy number fragments of DNA from the human, rat and chicken, and we have isolated a rat clone which also contains Dl5Kzl and Gdc-1 separated by about 3.5 kb. This conservation of the gene and its linkage relationship suggests that Dl5Kzl has an important cellular function; however further understanding will require analysis of the Dl5Kzl protein. The significance of the two structurally unrelated genes having such close linkage and being independently regulated may be that this region on chromosome 15 is a domain for ubiquitously expressed genes. We predict that other genes with ubiquitous expression are also located in this region. |
