| First Author | Cox DR | Year | 1984 |
| Journal | Cytogenet Cell Genet | Volume | 37 (1-4) |
| Pages | 443 (Abstr.) (399-616) | Mgi Jnum | J:12273 |
| Mgi Id | MGI:60522 | Citation | Cox DR, et al. (1984) Assignment of the murine histone genes to mouse Chromosome 13 (Abstracts of meeting presentations: Human gene mapping 7, Los Angeles Conference (1983) Seventh International Workshop on Human Gene Mapping). Cytogenet Cell Genet 37 (1-4):443 (Abstr.) (399-616) |
| abstractText | Full text of Abstract: Assignment of murine histone genes to mouse chromosome 13. D.R. Cox,1,4 V.E. Groppi,2 D. Bieber,4 D.B. Sittman,5 P. Coffino,3,4 and W.F. Marzluff5. Departments of 1Pediatrics, 2Pharmacology, 3Microbiology, and 4Biochemistry, University of California, San Francisco, CA, and 5Department of Chemistry, Florida State University, Tallahassee, FL A genomic mouse DNA clone which contains several different histone genes has previously been isolated from a library of mouse sperm DNA cloned in gamma phage. This clone, MM221, contains two regions which are complementary to sea urchin and drosophila H3 genes, as well as regions complementary to H2b, H2a, and H4 genes. Sequence analyses of the two H3 genes and the single H2b gene from MM221 have revealed that these genes correspond to H3.1, H3.2, and H2b.1 histone variants, respectively. Northern blot analyses have indicated that these genes are actively transcribed in mouse cells. We have isolated a 320 bp DNA fragment from the 3' non-coding region flanking the MM221 H3.2 gene and have used this fragment as a probe in association with 9 Chinese hamster-mouse and 4 rat-mouse somatic cell hybrids segregating mouse chromosomes, to determine the chromosomal origin of MM221. Southern blot analyses under conditions such that the probe did not react with Chinese hamster or rat DNA, in conjunction with isozyme and karyotype analyses, indicate that MM221 maps to mouse chromosome 13. |
