| First Author | Triarhou LC | Year | 1998 |
| Journal | Arch Histol Cytol | Volume | 61 |
| Issue | 3 | Pages | 233-42 |
| PubMed ID | 9756100 | Mgi Jnum | J:49935 |
| Mgi Id | MGI:1289603 | Doi | 10.1679/aohc.61.233 |
| Citation | Triarhou LC, et al. (1998) MAP2 and GAP-43 expression in normal and weaver mouse cerebellum: correlative immunohistochemical and in situ hybridization studies. Arch Histol Cytol 61(3):233-42 |
| abstractText | MAP2 is a major microtubule-associated brain protein, selectively localized in dendrites; growth-associated phosphoprotein GAP-43 is a neuron-specific protein associated with axonal outgrowth. In adult cerebellum, both of these proteins and their corresponding RNA transcripts are most strongly expressed by granule cells. Using immunocytochemistry with antibodies and in situ hybridization histochemistry with [32P] labeled oligonucleotide probes, we examined the cellular localization of MAP2, GAP-43 and their mRNAs in the cerebellum of control and weaver (wv/wv) mutant mice, which exhibit massive granule cell death. In wild-type (+/+) mice, MAP2 immuno-reactivity was seen in neuronal somata and dendrites of the granule cell layer; GAP-43 immuno-reactivity was present in molecular layer, corresponding to the distribution of parallel fibres. Tran-scripts encoding MAP2 and GAP-43 were localized in the layer of the granule cell somata. In hetero-zygous weaver mice (wv/+), which feature an intermediate degree of granule cell loss, MAP2 im-munoreactivity was localized in the granular layer, and the pattern of GAP-43 immunostaining was also similar to +/+, the only difference being a thinner molecular layer. Heterozygotes had an anatomical pattern of MAP2 and GAP-43 mRNA hybridization qualitatively similar to that of the wild-type with some deviations in signal intensity. In homozygous weaver mutants (wv/wv), MAP2 immunoreactivity was extremely weak in the area beneath Purkinje cells and a certain GAP-43 immunoreactivity was seen in the upper part of cerebellar cortex. Hybridization signals for MAP2 and GAP-43 mRNAs were minimal. The reported alterations in regional pattern of MAP2 and GAP-43 expression in mutant mice offer a molecular correlate of dendritic and axonal protein gene tran-scription pertinent to the neuropathological manifestations of certain forms of heredodegenerative ataxia. |
