| First Author | Bachmann MF | Year | 1997 |
| Journal | J Virol | Volume | 71 |
| Issue | 10 | Pages | 7253-7 |
| PubMed ID | 9311799 | Mgi Jnum | J:42955 |
| Mgi Id | MGI:1096777 | Doi | 10.1128/jvi.71.10.7253-7257.1997 |
| Citation | Bachmann MF, et al. (1997) Antiviral immune responses in Itk-deficient mice. J Virol 71(10):7253-7 |
| abstractText | Mice lacking Itk, a T-cell-specific protein tyrosine kinase, have reduced numbers of T cells and reduced responses to allogeneic major histocompatibility molecules. This study analyzed antiviral immune responses in mice deficient for Itk. Primary cytotoxic T-lymphocyte (CTL) responses were analyzed after infection with lymphocytic choriomeningitis virus (LCMV), vaccinia virus (VV), and vesicular stomatitis virus (VSV). Ex vivo CTL activity was consistently reduced by a factor of two to six for the different viruses. CTL responses after restimulation in vitro were similarly reduced unless exogenous cytokines were added. In the presence of interleukin-2 or concanavalin A supernatant, Itk-deficient and control mice responded similarly. Interestingly, while LCMV was completely eliminated by day 8 in both Itk-deficient and control mice, VV cleared from itk-/- mice with delayed kinetics. Antibody responses were evaluated after VSV infection. Both the T-cell-independent neutralizing immunoglobulin M (IgM) and the T-cell-dependent IgG responses were similar in Itk-deficient and control mice. Taken together, the results show that CTL responses are reduced in the absence of Itk whereas antiviral B-cell responses are not affected. |
