| First Author | Peng W | Year | 2023 |
| Journal | Cell Discov | Volume | 9 |
| Issue | 1 | Pages | 16 |
| PubMed ID | 36746933 | Mgi Jnum | J:370812 |
| Mgi Id | MGI:8235246 | Doi | 10.1038/s41421-022-00498-9 |
| Citation | Peng W, et al. (2023) Adenosine-independent regulation of the sleep-wake cycle by astrocyte activity. Cell Discov 9(1):16 |
| abstractText | Astrocytes play a crucial role in regulating sleep-wake behavior, and adenosine signaling is generally thought to be involved. Here we show multiple lines of evidence supporting that modulation of the sleep-wake behavior by astrocyte Ca(2+) activity could occur without adenosine signaling. In the basal forebrain and the brainstem, two brain regions that are known to be essential for sleep-wake regulation, chemogenetically-induced astrocyte Ca(2+) elevation significantly modulated the sleep-wake cycle. Although astrocyte Ca(2+) level positively correlated with the amount of extracellular adenosine, as revealed by a genetically encoded adenosine sensor, we found no detectable change in adenosine level after suppressing astrocyte Ca(2+) elevation, and transgenic mice lacking one of the major extracellular ATP-adenosine conversion enzymes showed similar extracellular adenosine level and astrocyte Ca(2+)-induced sleep modulation. Furthermore, astrocyte Ca(2+) is dependent primarily on local neuronal activity, causing brain region-specific regulation of the sleep-wake cycle. Thus, neural activity-dependent astrocyte activity could regulate the sleep-wake behavior independent of adenosine signaling. |
