| First Author | Liu L | Year | 2025 |
| Journal | STAR Protoc | Volume | 6 |
| Issue | 1 | Pages | 103523 |
| PubMed ID | 39705143 | Mgi Jnum | J:373055 |
| Mgi Id | MGI:8251030 | Doi | 10.1016/j.xpro.2024.103523 |
| Citation | Liu L, et al. (2025) Protocol for in vitro observation of HDAC4 condensation during induced cardiac reprogramming. STAR Protoc 6(1):103523 |
| abstractText | Here, we present a protocol to monitor and stimulate cardiac reprogramming via the disruption of Hdac4 condensates mediated by PC14-3-3 activation. We describe steps for inducing cardiac reprogramming in vitro, validating condensate formation ability of Hdac4, and observing Hdac4 condensates during reprogramming. We then detail procedures for disrupting these condensates using PC14-3-3 activation strategies. For complete details on the use and execution of this protocol, please refer to Liu et al.(1). |
