| First Author | Yuan J | Year | 2023 |
| Journal | Bone Res | Volume | 11 |
| Issue | 1 | Pages | 46 |
| PubMed ID | 37607905 | Mgi Jnum | J:374325 |
| Mgi Id | MGI:8259491 | Doi | 10.1038/s41413-023-00284-7 |
| Citation | Yuan J, et al. (2023) alpha-parvin controls chondrocyte column formation and regulates long bone development. Bone Res 11(1):46 |
| abstractText | Endochondral ossification requires proper control of chondrocyte proliferation, differentiation, survival, and organization. Here we show that knockout of alpha-parvin, an integrin-associated focal adhesion protein, from murine limbs causes defects in endochondral ossification and dwarfism. The mutant long bones were shorter but wider, and the growth plates became disorganized, especially in the proliferative zone. With two-photon time-lapse imaging of bone explant culture, we provide direct evidence showing that alpha-parvin regulates chondrocyte rotation, a process essential for chondrocytes to form columnar structure. Furthermore, loss of alpha-parvin increased binucleation, elevated cell death, and caused dilation of the resting zones of mature growth plates. Single-cell RNA-seq analyses revealed alterations of transcriptome in all three zones (i.e., resting, proliferative, and hypertrophic zones) of the growth plates. Our results demonstrate a crucial role of alpha-parvin in long bone development and shed light on the cellular mechanism through which alpha-parvin regulates the longitudinal growth of long bones. |
