| First Author | Journot RP | Year | 2025 |
| Journal | Dev Cell | Volume | 60 |
| Issue | 19 | Pages | 2576-2593.e6 |
| PubMed ID | 40592344 | Mgi Jnum | J:373097 |
| Mgi Id | MGI:8251097 | Doi | 10.1016/j.devcel.2025.06.007 |
| Citation | Journot RP, et al. (2025) Conserved signals control self-organization and symmetry breaking of murine bilayered epithelia during development and regeneration. Dev Cell 60(19):2576-2593.e6 |
| abstractText | Organ development relies on molecular cues that guide stem cells to differentiate within precise spatial arrangements. After injury, restoring these patterns is key for regeneration. Yet, how tissue geometry shapes cell fate remains unclear. This study employs a comprehensive approach using in vitro organoids, ex vivo embryonic tissue explants, and single-cell quantitative imaging to investigate cell fate acquisition in four murine bilayered epithelia during development and regeneration. The findings identify that tissue architecture serves as the primary driver of cell fate decisions, with symmetry breaking initiated during early cell internalization. Genetic and pharmacological analyses demonstrate that Hippo/YAP and Notch signaling pathways coordinate to link tissue structure with differentiation outcomes. This study identifies the inherent capacity of stem cells to self-organize into multicellular structures, where the precise position of each differentiated cell is critical to instruct their differentiation choices during embryonic development and regeneration. |
