| First Author | Krentz NAJ | Year | 2018 |
| Journal | Stem Cell Reports | Volume | 11 |
| Issue | 6 | Pages | 1551-1564 |
| PubMed ID | 30540962 | Mgi Jnum | J:376163 |
| Mgi Id | MGI:8269044 | Doi | 10.1016/j.stemcr.2018.11.008 |
| Citation | Krentz NAJ, et al. (2018) Single-Cell Transcriptome Profiling of Mouse and hESC-Derived Pancreatic Progenitors. Stem Cell Reports 11(6):1551-1564 |
| abstractText | Human embryonic stem cells (hESCs) are a potential unlimited source of insulin-producing beta cells for diabetes treatment. A greater understanding of how beta cells form during embryonic development will improve current hESC differentiation protocols. All pancreatic endocrine cells, including beta cells, are derived from Neurog3-expressing endocrine progenitors. This study characterizes the single-cell transcriptomes of 6,905 mouse embryonic day (E) 15.5 and 6,626 E18.5 pancreatic cells isolated from Neurog3-Cre; Rosa26(mT/mG) embryos, allowing for enrichment of endocrine progenitors (yellow; tdTomato + EGFP) and endocrine cells (green; EGFP). Using a NEUROG3-2A-eGFP CyT49 hESC reporter line (N5-5), 4,462 hESC-derived GFP+ cells were sequenced. Differential expression analysis revealed enrichment of markers that are consistent with progenitor, endocrine, or previously undescribed cell-state populations. This study characterizes the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors and serves as a resource (https://lynnlab.shinyapps.io/embryonic_pancreas) for improving the formation of functional beta-like cells from hESCs. |
