| Experiment Id | GSE252591 | Name | HuR promotes triglyceride synthesis and intestinal fat absorption |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2025-01-23 |
| description | Triacylglyceride (TAG) synthesis in the small intestine determines the absorption of dietary fat, but the mechanisms underlying are largely unknown. Here, we report that the RNA-binding protein HuR (ELAVL1) promotes TAG synthesis in the small intestine. HuR associates with the 3' UTR of Dgat2 mRNA and the introns 1 of Mgat2 pre-mRNA. Association of HuR with Dgat2 3' UTR stabilizes Dgat2 mRNA, while association of HuR with intron 1 of Mgat2 pre-mRNA promotes the processing of Mgat2 pre-mRNA. Intestinal epithelium-specific HuR knockout reduces the expression of DGAT2 and MGAT2, thereby reducing the dietary fat absorption through TAG synthesis and mitigating high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) and obesity. Our findings highlight a critical role of HuR in promoting dietary fat absorption. To investigate the function of HuR in intestinal fat absorption, we generated a conditional intestinal epithelium-specific HuR knockout (cKO) mouse. RNA was extracted from the proximal jejunum in HuR cKO and their wild-type (WT) littermates. We then performed gene expression profiling analysis using data obtained from RNA-seq of 5 WT and cKO mice. Comparative gene expression profiling analysis of RNA-seq data for cKO mice and its WT littermates. |
