| Experiment Id | GSE112270 | Name | Expansion of tolerogenic dendritic cells in neonatal lungs programs lymph node maturation, but impairs T cell proliferation |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2025-02-07 |
| description | Immune responses to pulmonary antigens are altered in neonates, possibly due to differences between neonatal and adult dendritic cells (DCs). Here we show that a novel population of DCs expressing intermediate levels of CD103 (CD103int DCs), transiently appears in the lungs and draining lymph nodes (LNs) between birth and 2 weeks of age. Like adult CD103+ DCs, CD103int DCs express XCR1 and CD205 and require the transcription factor, BATF3. However, CD103int DCs are constitutively activated and express high levels of CD40, CCR7 and PDL-1, independently of microbial exposure or MyD88-dependent signaling. Importantly, CD103int DCs spontaneously migrate to the draining LN, promote the maturation of lymph node stromal cells and increase the size and cellularity of the LN, but paradoxically dampen CD4 T cell proliferation. Our results suggest that CD103int DCs are developmentally programmed to promote LN maturation and dampen T cell responses in neonates. 3 samples with 2 replicates each. Each sample is one of the conventional Dendritic cell subsets found in mice neonatal lung (as defined in this publication): CD11b+ SIRPa+, CD103Hi XCR1+, CD103 int XCR1+ |
