Experiment Id | GSE60489 | Name | Global heart transcript data from fasted male BXD strains on chow or high fat diet |
Experiment Type | transcription profiling by array | Study Type | Baseline |
Source | GEO | Curation Date | 2022-03-14 |
description | Transcript data from heart tissue from fasted-state male BXD strains on chow or high fat diet We used microarrays to compare the heart expression differences across the BXD strain family and across two diverse diets 29-week-old male mice were fasted overnight (6pm-9am), anesthetized under isoflurane, and perfused, then hearts were snap-frozen in liquid nitrogen for RNA extraction and RNEasy cleanup. Each dietary and strain cohort consisted of ~5 animals which were prepared independently then pooled evenly by ~5 µg RNA before the Affymetrix arrays were run. |