| Experiment Id | E-TABM-367 | Name | Transcription profiling by array of limb buds from E12.5 POR knock-out mice |
| Experiment Type | transcription profiling by array | Study Type | WT vs. Mutant |
| Source | ArrayExpress | Curation Date | 2018-04-16 |
| description | POR is the obligate electron donor for all microsomal P450 enzymes. POR was conditionally knocked out during limb development at E9.5. The experiment was set up to answer two questions. First, to investigate which genes are differentially expressed in POR deficient limb buds and are influenced by a metabolically inactive cytochrome P450 system. Second, to assess which P450 enzymes are expressed at all at E12.5 during limb development and therefore which metabolic pathways involving this group of enzymes are present at this stage. Differentially expressed genes were analysed by comparing 5 wild type control samples with 5 conditional knock out samples (whole forelimb buds each). Present calls of control samples were screened for annotated P450s to establish a list of expressed P450 enzymes at E12.5. |
