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HT Experiment :

Experiment Id  E-GEOD-49218 Series Id  GSE49218
Name  Gene regulation by CBP-p300 in the lens placode - E10.5 Experiment Type  transcription profiling by array
Study Type  WT vs. Mutant Source  ArrayExpress
Curation Date  2017-10-10
description  Wild type or CBP/p300 fx/fx; Pax6-Cre-positive (CBP/p300-/-) surface ectoderm from E10.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.
  • variables:
  • genotype

1 Publications

Trail: HTExperiment

6 Samples

Trail: HTExperiment