Experiment Id | E-GEOD-49227 | Series Id | GSE49227 |
Name | Gene regulation by Pax6 in the lens placode - E9.5 (1) | Experiment Type | transcription profiling by array |
Study Type | WT vs. Mutant | Source | ArrayExpress |
Curation Date | 2019-05-28 |
description | Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays. Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues (left and right eyes) from each embryo. |