Experiment Id | E-GEOD-33138 | Series Id | GSE33138 |
Name | Gene expression of Tg 3xFLAG-Nanos2-deltaN10 mouse male gonad | Experiment Type | transcription profiling by array |
Study Type | WT vs. Mutant | Source | ArrayExpress |
Curation Date | 2019-01-15 |
description | To investigate the biochemical function of NANOS2, we performed expression microarray analysis of the embryonic male gonad of Nanos2 hetero, Nanos2 KO, and Nanos2 KO_Tg 3xFLAG-tagged Nanos2-deltaN10, which is truncated form of Nanos2 in the N-terminal region, transgenic mice. 3xFLAG-tagged Nanos2-deltaN10 protein function was validated by rescue experiment in the Nanos2-null male gonad of mouse embryo at E14.5. Biological duplicates were examined at each genotype, Nanos2 hetero, Nanos2 KO, and Nanos2 KO_Tg 3xFLAG-tagged Nanos2-deltaN10 for each experiment. |