Experiment Id | E-GEOD-24822 | Series Id | GSE24822 |
Name | Small non-coding RNA expression in murine germ cells | Experiment Type | RNA-Seq |
Study Type | Baseline | Source | ArrayExpress |
Curation Date | 2019-04-04 |
description | In order to monitor the changes in small RNAs expression during mouse spermatogenesis. Type A spermatogonia, pachytene spermatocytes and round spermatids were isolated following collagenase treatment of testes and trypsin digestion of isolated seminiferous tubules using unit gravity sedimentation in a bovine serum albumin gradient. The small RNA fraction (18-36nt) was cloned and sequenced from total RNA of each cell type. Three types of mouse germ cells small RNAs were used for high throughput sequencing analysis |