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HT Experiment :

Experiment Id  E-GEOD-29151 Series Id  GSE29151
Name  Bglu3 congenic mice - gene expression in liver Experiment Type  transcription profiling by array
Study Type  WT vs. Mutant Source  ArrayExpress
Curation Date  2018-08-08
description  A congenic line was constructed by introgressing a C3H chromosome 1 region harboring Bglu3 into C57BL/6 apoE-/- background. RNA was extracted from liver using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the liver of Bglu3 congenic mice and C57BL/6 apoE-deficient mouse strains fed a western diet. 2 groups of mice were studied: C57BL/6 apoE-/- control mice and Bglu3 congenic mice. After fed a Western diet for 12 weeks, liver was harvested and total RNA prepared.
  • variables:
  • genotype

1 Publications

Trail: HTExperiment

2 Samples

Trail: HTExperiment