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HT Experiment :

Experiment Id  E-GEOD-29149 Series Id  GSE29149
Name  Ath29 congenic mice - gene expression in aorta Experiment Type  transcription profiling by array
Study Type  WT vs. Mutant Source  ArrayExpress
Curation Date  2018-08-21
description  A congenic mouse line was constructed by introgressing a C3H chromosome 9 region harboring Ath29 into the C57BL/6 apoE-deficient background. RNA was extracted from aorta using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the aorta of Ath29 congenic mice and C57BL/6 apoE-deficient mouse strains fed a chow or western diet. 4 groups of mice were studied: C57BL/6 apoE-/- control mice and Ath29 congenic mice fed a chow or Western diet. After fed a Western diet for 2 weeks, aorta was harvested and total RNA prepared.
  • variables:
  • genotype

1 Publications

Trail: HTExperiment

4 Samples

Trail: HTExperiment