| Experiment Id | E-GEOD-55060 | Series Id | GSE55060 |
| Name | SCML2 Establishes the Male Germline Epigenome | Experiment Type | RNA-Seq |
| Study Type | WT vs. Mutant | Source | ArrayExpress |
| Curation Date | 2019-04-04 |
| description | Gametogenesis is dependent on the expression of germline-specific genes. However, it remains unknown how the germline epigenome is distinctly established from that of somatic lineages. Here we show that genes commonly expressed in somatic lineages and spermatogenesis-progenitor cells undergo repression in a genome-wide manner in late stages of the male germline and identify underlying mechanisms. SCML2, a germline-specific subunit of a Polycomb repressive complex 1 (PRC1), establishes the unique epigenome of the male germline through two distinct antithetical mechanisms. SCML2 works with PRC1 and promotes RNF2-dependent ubiquitination of H2A, thereby marking somatic/progenitor genes on autosomes for repression. Paradoxically, SCML2 also prevents RNF2-dependent ubiquitination of H2A on sex chromosomes during meiosis, thereby enabling unique epigenetic programming of sex chromosomes for male reproduction. Our results reveal divergent mechanisms involving a shared regulator by which the male germline epigenome is distinguished from that of the soma and progenitor cells. RNA-seq and ChIP-seq analyses using wild-type and Scml2 KO spermatogenic cells. |
