Experiment Id | E-GEOD-54796 | Series Id | GSE54796 |
Name | Gene regulation by Sox9 in the conjunctival epithelium | Experiment Type | transcription profiling by array |
Study Type | WT vs. Mutant | Source | ArrayExpress |
Curation Date | 2018-01-25 |
description | Wild type or Sox9 fx/fx; Le-Cre-positive (Sox9-/-) conjunctival epithelium from the temporal, nasal and dorsal region of E14.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina MouseWG-6 v2.0 bead arrays. Three wild type and three Sox9 knockout embryos were used. cDNA obtained from each region of the conjunctival epithelium was pooled and used for microarray analysis. Statistical analysis was performed using the values from the 25-50 beads on each array. |