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HT Experiment :

Experiment Id  E-GEOD-54796 Series Id  GSE54796
Name  Gene regulation by Sox9 in the conjunctival epithelium Experiment Type  transcription profiling by array
Study Type  WT vs. Mutant Source  ArrayExpress
Curation Date  2018-01-25
description  Wild type or Sox9 fx/fx; Le-Cre-positive (Sox9-/-) conjunctival epithelium from the temporal, nasal and dorsal region of E14.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina MouseWG-6 v2.0 bead arrays. Three wild type and three Sox9 knockout embryos were used. cDNA obtained from each region of the conjunctival epithelium was pooled and used for microarray analysis. Statistical analysis was performed using the values from the 25-50 beads on each array.
  • variables:
  • anatomical structure,
  • genotype

1 Publications

Trail: HTExperiment

6 Samples

Trail: HTExperiment