Experiment Id | E-GEOD-49442 | Series Id | GSE49442 |
Name | miRNA expression profiling in testis of mouse inter-subspecific hybrids | Experiment Type | transcription profiling by array |
Study Type | Baseline | Source | ArrayExpress |
Curation Date | 2018-08-10 |
description | MiRNA expression profiling on 14.5 day old testis from sterile (PWD x B6)F1, (PWK x B6)F1 and fertile (B6 x PWD)F1 inter-subspecific hybrids were performed to study the genome wide variation in miRNA expression between different inter-subspecific hybrids during meiosis. The total RNA (120 ng) samples were extracted from 14.5 days juvenile testes (pool of 4 samples), converted to cDNA using the Affymetrix 3 IVT Express Kit and hybridized to Affymetrix Mouse miRNA Gene 1.0ST GeneChips (core facility of the Institute of Molecular Genetics AS CR, Prague, Czech Rep.) |